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NOX2/gp91phox Rabbit mAb (A19701)

Publication (1) Datasheet

ABclonal:Western blot - NOX2/gp91phox Rabbit mAb (A19701)

Western blot analysis of extracts of various cell lines, using NOX2/gp91phox antibody (A19701) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

ABclonal:Western blot - NOX2/gp91phox Rabbit mAb (A19701)

Western blot analysis of extracts of various cell lines, using NOX2/gp91phox antibody (A19701) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product nameNOX2/gp91phox Rabbit mAb
Catalog No.A19701
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]
ImmunogenA synthesized peptide derived from human NOX2/gp91phox .
SequenceEmail for sequence
Gene ID1536
Swiss protP04839
SynonymsAMCBX2; CGD; GP91-1; GP91-PHOX; GP91PHOX; IMD34; NOX2; p91-PHOX; CYBB
Calculated MW65kDa
Observed MW65KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMouse spleen, Mouse heart, Rat lung, Rat spleen, HepG2, Raji, HL-60, Mouse lung
Cellular locationCell membrane, Multi-pass membrane protein
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - NOX2/gp91phox Rabbit mAb (A19701) }

Western blot - NOX2/gp91phox Rabbit mAb (A19701)

Western blot analysis of extracts of various cell lines, using NOX2/gp91phox antibody (A19701) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
ABclonal:Western blot - NOX2/gp91phox Rabbit mAb (A19701) }

Western blot - NOX2/gp91phox Rabbit mAb (A19701)

Western blot analysis of extracts of various cell lines, using NOX2/gp91phox antibody (A19701) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

* For research use only. Not for therapeutic or diagnostic purposes.

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