Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

NODAL Rabbit pAb (A9902)

Review (1)Publication (1) Datasheet SDS COA

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - NODAL Rabbit pAb (A9902)

Western blot analysis of extracts of various cell lines, using NODAL antibody (A9902) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Review

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Overview

Product name	NODAL Rabbit pAb
Catalog No.	A9902
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which regulates early embryonic development. This protein is required for maintenance of human embryonic stem cell pluripotency and may play a role in human placental development. Mutations in this gene are associated with heterotaxy, a condition characterized by random orientation of visceral organs with respect to the left-right axis.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 198-347 of human NODAL (NP_060525.3).
Sequence	SNLSQEQRQLGGSTLLWEAESSWRAQEGQLSWEWGKRHRRHHLPDRSQLCRKVKFQVDFNLIGWGSWIIYPKQYNAYRCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLDHHKDMIVEECGCL
Gene ID	4838
Swiss prot	Q96S42
Synonyms	HTX5
Calculated MW	40kDa
Observed MW	44kDa

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting
Positive samples	SH-SY5Y, HepG2, 293T, Rat liver
Cellular location	Secreted
Customer validation	IHC(Human glioma tissue) WB(Homo sapiens) IF(Homo sapiens) IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

NODAL Rabbit pAb images

Western blot - NODAL Rabbit pAb (A9902)

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Related Targets

Based on existing publications, the following genes are closely related to research on NODAL. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to NODAL. (Distance between topics and target gene indicate popularity.) NODAL

* Data provided by citexs.com, for reference only.