Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

NODAL Rabbit pAb (A9902)

Review (1)Publication (1) Datasheet SDS COA

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition

Reactivity:Human, Rat

ABclonal:Western blot - NODAL Rabbit pAb (A9902)

Western blot analysis of extracts of various cell lines, using NODAL antibody (A9902) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Review
ABclonal: review for NODAL Rabbit pAb(A9902)

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    Overview

    Product nameNODAL Rabbit pAb
    Catalog No.A9902
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG

    Background

    This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which regulates early embryonic development. This protein is required for maintenance of human embryonic stem cell pluripotency and may play a role in human placental development. Mutations in this gene are associated with heterotaxy, a condition characterized by random orientation of visceral organs with respect to the left-right axis.

    Immunogen information

    ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 198-347 of human NODAL (NP_060525.3).
    SequenceSNLSQEQRQLGGSTLLWEAESSWRAQEGQLSWEWGKRHRRHHLPDRSQLCRKVKFQVDFNLIGWGSWIIYPKQYNAYRCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLDHHKDMIVEECGCL
    Gene ID4838
    Swiss protQ96S42
    SynonymsHTX5
    Calculated MW40kDa
    Observed MW44kDa

    Applications

    ReactivityHuman, Rat
    Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition
    Recommended dilution
    • WB 1:500 - 1:2000
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
    Application keyWestern blotting    
    Positive samplesSH-SY5Y, HepG2, 293T, Rat liver
    Cellular locationSecreted
    Customer validation

    IHC(Human glioma tissue)

    WB(Homo sapiens)

    IF(Homo sapiens)

    IHC(Homo sapiens)

    Documents

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    Lot number

    Research Area

    NODAL Rabbit pAb images

    ABclonal:Western blot - NODAL Rabbit pAb (A9902)}

    Western blot - NODAL Rabbit pAb (A9902)

    Western blot analysis of extracts of various cell lines, using NODAL antibody (A9902) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

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    Related Targets

    Based on existing publications, the following genes are closely related to research on NODAL. (Numbers indicate number of published articles where both targets appear.)

    1/

    Related Research Area

    Based on existing publications, the following research topics are related to NODAL. (Distance between topics and target gene indicate popularity.) NODAL

    * Data provided by citexs.com, for reference only.

    Publishing research using A9902? Please let us know so that we can cite the reference in this datasheet.

    * For research use only. Not for therapeutic or diagnostic purposes.

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