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NMDAR2A Rabbit pAb (A0924)

Review (1)Publications (3) Datasheet

ABclonal:Western blot - NMDAR2A Rabbit pAb (A0924)

Western blot analysis of extracts of various cell lines, using NMDAR2A antibody (A0924) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - NMDAR2A Rabbit pAb (A0924)

Immunohistochemistry of paraffin-embedded rat brain using NMDAR2A antibody (A0924) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - NMDAR2A Rabbit pAb (A0924)

Immunofluorescence analysis of H9C2 cells using NMDAR2A Rabbit pAb (A0924) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NMDAR2A Rabbit pAb (A0924)

Immunofluorescence analysis of L929 cells using NMDAR2A Rabbit pAb (A0924) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for NMDAR2A Rabbit pAb(A0924)

Overview

Product nameNMDAR2A Rabbit pAb
Catalog No.A0924
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1130-1400 of human NMDAR2A (NP_000824.1).
SequenceLDPPQFVENVTLPENVDFPDPYQDPSENFRKGDSTLPMNRNPLHNEEGLSNNDQYKLYSKHFTLKDKGSPHSETSERYRQNSTHCRSCLSNMPTYSGHFTMRSPFKCDACLRMGNLYDIDEDQMLQETGNPATGEQVYQQDWAQNNALQLQKNKLRISRQHSYDNIVDKPRELDLSRPSRSISLKDRERLLEGNFYGSLFSVPSSKLSGKKSSLFPQGLEDSKRSKSLLPDHTSDNPFLHSHRDDQRLVIGRCPSDPYKHSLPSQAVNDSY
Gene ID2903
Swiss protQ12879
SynonymsEPND; FESD; GluN2A; LKS; NMDAR2A; NR2A; GRIN2A; NMDA 2A
Calculated MW144kDa/165kDa
Observed MW180KDa
ReactivityMouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain, Rat brain
Cellular locationCell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Customer validation

(mouse brain)

WB(Mus musculus, Rattus norvegicus)

ABclonal:Western blot - NMDAR2A Rabbit pAb (A0924) }

Western blot - NMDAR2A Rabbit pAb (A0924)

Western blot analysis of extracts of various cell lines, using NMDAR2A antibody (A0924) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - NMDAR2A Rabbit pAb (A0924) }

Immunohistochemistry - NMDAR2A Rabbit pAb (A0924)

Immunohistochemistry of paraffin-embedded rat brain using NMDAR2A antibody (A0924) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - NMDAR2A Rabbit pAb (A0924) }

Immunofluorescence - NMDAR2A Rabbit pAb (A0924)

Immunofluorescence analysis of H9C2 cells using NMDAR2A Rabbit pAb (A0924) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NMDAR2A Rabbit pAb (A0924) }

Immunofluorescence - NMDAR2A Rabbit pAb (A0924)

Immunofluorescence analysis of L929 cells using NMDAR2A Rabbit pAb (A0924) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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