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NLRP3 Rabbit pAb (A5652)

Publications (30) Datasheet

ABclonal:Western blot - NLRP3 Rabbit pAb (A5652)

Western blot analysis of extracts of various cell lines, using NLRP3 Rabbit pAb (A5652) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - NLRP3 Rabbit pAb (A5652)

Western blot analysis of extracts of Rat lung, using NLRP3 antibody (A5652) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - NLRP3 Rabbit pAb (A5652)

Immunohistochemistry of paraffin-embedded human liver cancer using NLRP3 antibody (A5652) at dilution of 1:50 (40x lens).

ABclonal:Immunohistochemistry - NLRP3 Rabbit pAb (A5652)

Immunohistochemistry of paraffin-embedded mouse liver using NLRP3 antibody (A5652) at dilution of 1:50 (40x lens).

ABclonal:Immunohistochemistry - NLRP3 Rabbit pAb (A5652)

Immunohistochemistry of paraffin-embedded rat lung using NLRP3 antibody (A5652) at dilution of 1:50 (40x lens).

ABclonal:Immunofluorescence - NLRP3 Rabbit pAb (A5652)

Immunofluorescence analysis of U2OS cells using NLRP3 antibody (A5652) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NLRP3 Rabbit pAb (A5652)

Immunofluorescence analysis of THP-1 cells using NLRP3 antibody (A5652) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NLRP3 Rabbit pAb (A5652)

Immunofluorescence analysis of RAW264.7 cells using NLRP3 antibody (A5652) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NLRP3 Rabbit pAb (A5652)

Immunofluorescence analysis of NIH/3T3 cells using NLRP3 antibody (A5652) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NLRP3 Rabbit pAb (A5652)

Immunofluorescence analysis of PC-12 cells using NLRP3 antibody (A5652) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameNLRP3 Rabbit pAb
Catalog No.A5652
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.
ImmunogenRecombinant protein of human NLRP3
SequenceEmail for sequence
Gene ID114548
Swiss protQ96P20
SynonymsNLRP3; AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCAS1; FCU; MWS; NALP3; PYPAF1
Calculated MW83kDa/105kDa/111kDa/112kDa/115kDa/118kDa
Observed MW110KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, Mouse lung, Rat lung
Cellular locationCytoplasm, Endoplasmic reticulum, Inflammasome, Nucleus, Secreted, cytosol
Customer validation

WB(Homo sapiens, Rattus norvegicus, Mus musculus)

IF(Mus musculus)

IHC(Mus musculus)

IHC(Homo sapiens,Mus musculus)

ABclonal:Western blot - NLRP3 Rabbit pAb (A5652) }

Western blot - NLRP3 Rabbit pAb (A5652)

Western blot analysis of extracts of various cell lines, using NLRP3 Rabbit pAb (A5652) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - NLRP3 Rabbit pAb (A5652) }

Western blot - NLRP3 Rabbit pAb (A5652)

Western blot analysis of extracts of Rat lung, using NLRP3 antibody (A5652) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - NLRP3 Rabbit pAb (A5652) }

Immunohistochemistry - NLRP3 Rabbit pAb (A5652)

Immunohistochemistry of paraffin-embedded human liver cancer using NLRP3 antibody (A5652) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - NLRP3 Rabbit pAb (A5652) }

Immunohistochemistry - NLRP3 Rabbit pAb (A5652)

Immunohistochemistry of paraffin-embedded mouse liver using NLRP3 antibody (A5652) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - NLRP3 Rabbit pAb (A5652) }

Immunohistochemistry - NLRP3 Rabbit pAb (A5652)

Immunohistochemistry of paraffin-embedded rat lung using NLRP3 antibody (A5652) at dilution of 1:50 (40x lens).
ABclonal:Immunofluorescence - NLRP3 Rabbit pAb (A5652) }

Immunofluorescence - NLRP3 Rabbit pAb (A5652)

Immunofluorescence analysis of U2OS cells using NLRP3 antibody (A5652) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NLRP3 Rabbit pAb (A5652) }

Immunofluorescence - NLRP3 Rabbit pAb (A5652)

Immunofluorescence analysis of THP-1 cells using NLRP3 antibody (A5652) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NLRP3 Rabbit pAb (A5652) }

Immunofluorescence - NLRP3 Rabbit pAb (A5652)

Immunofluorescence analysis of RAW264.7 cells using NLRP3 antibody (A5652) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NLRP3 Rabbit pAb (A5652) }

Immunofluorescence - NLRP3 Rabbit pAb (A5652)

Immunofluorescence analysis of NIH/3T3 cells using NLRP3 antibody (A5652) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NLRP3 Rabbit pAb (A5652) }

Immunofluorescence - NLRP3 Rabbit pAb (A5652)

Immunofluorescence analysis of PC-12 cells using NLRP3 antibody (A5652) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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