Products > Catalog Antibodies > Polyclonal Antibodies

NLRP3 Rabbit pAb (A5652)

Publications (65) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Mouse, Rat

ABclonal:Western blot - NLRP3 Rabbit pAb (A5652)

Western blot analysis of extracts of 293T cells, using NLRP3 antibody (A5652) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - NLRP3 Rabbit pAb (A5652)

Western blot analysis of extracts of Rat lung, using NLRP3 antibody (A5652) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameNLRP3 Rabbit pAb
Catalog No.A5652
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-210 of human NLRP3 (NP_004886.3).
SequenceRVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFD
Gene ID114548
Swiss protQ96P20
SynonymsNLRP3; AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCAS1; FCU; MWS; NALP3; PYPAF1
Calculated MW83kDa/105kDa/111kDa/112kDa/115kDa/118kDa
Observed MW110KDa
ReactivityMouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samples293T, Rat lung
Cellular locationCytoplasm, Endoplasmic reticulum, Inflammasome, Nucleus, Secreted, cytosol
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Danio rerio)

IF(Mus musculus, Rattus norvegicus)

IHC(Mus musculus)

IHC(Homo sapiens,Mus musculus)

IHC(Mus musculus)

IF(Mus musculus)

IF(Mus musculus)

WB(Mus musculus)

ABclonal:Western blot - NLRP3 Rabbit pAb (A5652)}

Western blot - NLRP3 Rabbit pAb (A5652)

Western blot analysis of extracts of 293T cells, using NLRP3 antibody (A5652) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - NLRP3 Rabbit pAb (A5652)}

Western blot - NLRP3 Rabbit pAb (A5652)

Western blot analysis of extracts of Rat lung, using NLRP3 antibody (A5652) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Inquire About This Product

Submit your question about A5652 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A5652? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order