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NLGN3 Rabbit pAb (A10310)

Datasheet

ABclonal:Western blot - NLGN3 Rabbit pAb (A10310)

Western blot analysis of extracts of various cell lines, using NLGN3 antibody (A10310) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product nameNLGN3 Rabbit pAb
Catalog No.A10310
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. Mutations in this gene may be associated with autism and Asperger syndrome. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
ImmunogenRecombinant protein of human NLGN3
SequenceEmail for sequence
Gene ID54413
Swiss protQ9NZ94
SynonymsNLGN3; HNL3
Calculated MW89kDa/91kDa/93kDa
Observed MW108kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:1000 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesU-87MG, K-562, Mouse brain, Mouse liver
Cellular locationCell junction, Cell membrane, Single-pass type I membrane protein, synapse
ABclonal:Western blot - NLGN3 Rabbit pAb (A10310) }

Western blot - NLGN3 Rabbit pAb (A10310)

Western blot analysis of extracts of various cell lines, using NLGN3 antibody (A10310) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

* For research use only. Not for therapeutic or diagnostic purposes.

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