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NF-kB p65/RelA Rabbit pAb (A2547)

Reviews (6)Publications (41) Datasheet

ABclonal:Western blot - NF-kB p65/RelA Rabbit pAb (A2547)

Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA Rabbit pAb (A2547) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)

Immunohistochemistry of paraffin-embedded human stomach using NF-kB p65/RelA Antibody (A2547) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)

Immunofluorescence analysis of C6 cells using NF-kB p65/RelA antibody (A2547) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)

Immunofluorescence analysis of L929 cells using NF-kB p65/RelA antibody (A2547) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)

Immunofluorescence analysis of U2OS cells using NF-kB p65/RelA antibody (A2547) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A2547)
Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A2547)
Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A2547)
Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A2547)
Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A2547)
Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A2547)

Overview

Product nameNF-kB p65/RelA Rabbit pAb
Catalog No.A2547
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 50-180 of human NF-kB p65/RelA (NP_068810.3).
SequenceRSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLS
Gene ID5970
Swiss protQ04206
SynonymsNFKB3; p65; NF-kB p65; RELA; CMCU
Calculated MW58kDa/59kDa/60kDa
Observed MW65KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, 293T, MCF7, Mouse lung, Mouse kidney, Rat lung, Rat kidney
Cellular locationCytoplasm, Nucleus
Customer validation

(RAW264.7, Mouse atherosclerosis arterial tissue, HCC827, U251, Rat H9c2 cells)

WB(Alpinia katsumadai Hayata, Mus musculus, Rattus norvegicus, Homo sapiens, Sus scrofa, Macaca mulatta, Gallus gallus, Schisandra chinensis)

IHC(Mus musculus, Rattus norvegicus)

IF(Homo sapiens, Mus musculus, Rattus norvegicus)

ChIP(Homo sapiens)

ABclonal:Western blot - NF-kB p65/RelA Rabbit pAb (A2547) }

Western blot - NF-kB p65/RelA Rabbit pAb (A2547)

Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA Rabbit pAb (A2547) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547) }

Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)

Immunohistochemistry of paraffin-embedded human stomach using NF-kB p65/RelA Antibody (A2547) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547) }

Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)

Immunofluorescence analysis of C6 cells using NF-kB p65/RelA antibody (A2547) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547) }

Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)

Immunofluorescence analysis of L929 cells using NF-kB p65/RelA antibody (A2547) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547) }

Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)

Immunofluorescence analysis of U2OS cells using NF-kB p65/RelA antibody (A2547) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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