Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

NF-kB p65/RelA Rabbit mAb (A19653)

Review (1)Publications (55) Datasheet SDS COA

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition

Reactivity:Human, Mouse, Rat, Monkey

ABclonal:Western blot - NF-kB p65/RelA Rabbit mAb (A19653)

Western blot analysis of extracts of various lysates, using NF-kB p65/RelA Rabbit mAb (A19653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit mAb (A19653)

Immunohistochemistry analysis of paraffin-embedded Human colon using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit mAb (A19653)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit mAb (A19653)

Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Rabbit mAb (A19653) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit mAb (A19653)

Immunofluorescence analysis of U-2 OS cells using NF-kB p65/RelA Rabbit mAb (A19653) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - NF-kB p65/RelA Rabbit mAb (A19653)

Chromatin immunoprecipitation analysis of extracts of HT-1080 treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes, using NF-kB p65/RelA Rabbit mAb antibody (A19653) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review
ABclonal: review for NF-kB p65/RelA Rabbit mAb(A19653)

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    Overview

    Product nameNF-kB p65/RelA Rabbit mAb
    Catalog No.A19653
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG
    CloneNo.ARC51086

    Background

    NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

    Immunogen information

    ImmunogenA synthetic peptide corresponding to a sequence within amino acids 450-551 of human NF-κB p65 (Q04206).
    SequenceLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS
    Gene ID5970
    Swiss protQ04206
    Synonymsp65; CMCU; NFKB3; AIF3BL3
    Calculated MW60kDa
    Observed MW65kDa

    Applications

    ReactivityHuman, Mouse, Rat, Monkey
    Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition
    Recommended dilution
    • WB 1:500 - 1:1000
    • IHC-P 1:50 - 1:200
    • IF/ICC 1:50 - 1:200
    • ChIP 1:50 - 1:200
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
    Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
    Positive samplesPC-12, Mouse spleen, Mouse lung
    Cellular locationCytoplasm, Nucleus
    Customer validation

    (RPMI-8226、PC-3、HepG2、Hela、U2-OS、Jurkat、MOLT-4)

    WB(Mus musculus, Homo sapiens, Rattus norvegicus)

    IF(Homo sapiens)

    IHC(Rattus norvegicus, A. baumannii)

    WB(Homo sapiens)

    IF(Rattus norvegicus, Mus musculus , Mus musculus)

    WB,IF(Rattus norvegicus, Mus musculus)

    IP(Mus musculus )

    (Rattus norvegicus)

    IHC(Mus musculus)

    IF/ICC(Homo sapiens)

    FC(Homo sapiens )

    Documents

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    Lot number

    Research Area

    NF-kB p65/RelA Rabbit mAb images

    ABclonal:Western blot - NF-kB p65/RelA Rabbit mAb (A19653)}

    Western blot - NF-kB p65/RelA Rabbit mAb (A19653)

    Western blot analysis of extracts of various lysates, using NF-kB p65/RelA Rabbit mAb (A19653) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit mAb (A19653)}

    Immunohistochemistry - NF-kB p65/RelA Rabbit mAb (A19653)

    Immunohistochemistry analysis of paraffin-embedded Human colon using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit mAb (A19653)}

    Immunohistochemistry - NF-kB p65/RelA Rabbit mAb (A19653)

    Immunohistochemistry analysis of paraffin-embedded Human tonsil using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit mAb (A19653)}

    Immunofluorescence - NF-kB p65/RelA Rabbit mAb (A19653)

    Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Rabbit mAb (A19653) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit mAb (A19653)}

    Immunofluorescence - NF-kB p65/RelA Rabbit mAb (A19653)

    Immunofluorescence analysis of U-2 OS cells using NF-kB p65/RelA Rabbit mAb (A19653) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Chromatin Immunoprecipitation - NF-kB p65/RelA Rabbit mAb (A19653)}

    Chromatin Immunoprecipitation - NF-kB p65/RelA Rabbit mAb (A19653)

    Chromatin immunoprecipitation analysis of extracts of HT-1080 treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes, using NF-kB p65/RelA Rabbit mAb antibody (A19653) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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    Related Targets

    Based on existing publications, the following genes are closely related to research on RELA. (Numbers indicate number of published articles where both targets appear.)

    1/

    Related Research Area

    Based on existing publications, the following research topics are related to RELA. (Distance between topics and target gene indicate popularity.) RELA

    * Data provided by citexs.com, for reference only.

    Publishing research using A19653? Please let us know so that we can cite the reference in this datasheet.

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