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[KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Publications (10) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat, Monkey

ABclonal:Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (A19653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (A19653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (A19653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunohistochemistry of paraffin-embedded rat spleen using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunohistochemistry of paraffin-embedded human lung cancer using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Rabbit mAb (A19653) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug NF-kB p65/RelA antibody (A19653). Western blot was performed from the immunoprecipitate using NF-kB p65/RelA antibody (A19653) at a dilition of 1:1000.

Overview

Product name[KO Validated] NF-kB p65/RelA Rabbit mAb
Catalog No.A19653
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
ImmunogenA synthesized peptide derived from human NF-κB p65.
SequenceEmail for sequence
Gene ID5970
Swiss protQ04206
SynonymsNFKB3; p65; NF-kB p65; RELA; CMCU
Calculated MW65kDa
Observed MW65KDa
ReactivityHuman, Mouse, Rat, Monkey
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesMCF7, 293T, Mouse spleen, Mouse liver, HeLa
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

IF(Homo sapiens)

ABclonal:Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653) }

Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (A19653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653) }

Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (A19653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653) }

Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (A19653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653) }

Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunohistochemistry of paraffin-embedded rat spleen using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653) }

Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunohistochemistry of paraffin-embedded human lung cancer using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653) }

Immunofluorescence - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Rabbit mAb (A19653) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653) }

Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug NF-kB p65/RelA antibody (A19653). Western blot was performed from the immunoprecipitate using NF-kB p65/RelA antibody (A19653) at a dilition of 1:1000.

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