Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

NF-kB p65/RelA Mouse mAb (A10609)

Reviews (3) Publications (50) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - NF-kB p65/RelA Mouse mAb (A10609)

Western blot analysis of various lysates using NF-kB p65/RelA Mouse mAb (A10609) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - NF-kB p65/RelA Mouse mAb (A10609)

Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Mouse mAb (A10609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NF-kB p65/RelA Mouse mAb (A10609)

Immunofluorescence analysis of C6 cells using NF-kB p65/RelA Mouse mAb (A10609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NF-kB p65/RelA Mouse mAb (A10609)

Immunofluorescence analysis of U2OS cells using NF-kB p65/RelA Mouse mAb (A10609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for NF-kB p65/RelA Mouse mAb(A10609)
Review
ABclonal: review for NF-kB p65/RelA Mouse mAb(A10609)
Review
ABclonal: review for NF-kB p65/RelA Mouse mAb(A10609)

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Overview

Product name NF-kB p65/RelA Mouse mAb
Catalog No. A10609
Host species Mouse
Purification method Affinity purification
Isotype IgG1
CloneNo. AMC0486

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant protein of human NF-kB p65
Sequence Email for sequence
Gene ID 5970
Swiss prot Q04206
Synonyms p65; CMCU; NFKB3; AIF3BL3; NF-kB p65/RelA
Calculated MW 60kDa
Observed MW 70kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa, Jurkat, HT-29, SGC-7901, NIH/3T3, Mouse liver
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Mononuclear macrophages, human cellsHomo sapiens, Mus musculus, Edwardsiella tarda, Mauremys sinensis, Cyprinus carpio, Rattus norvegicus, Homo sapiens, Sus scrofa)

IF (human cellsMus musculus, Mus musculus, Homo sapiens, Rattus norvegicus)

IHC (Rattus norvegicus, Mus musculus, Sus scrofa)

Co-IP (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

NF-kB p65/RelA Mouse mAb images

ABclonal:Western blot - NF-kB p65/RelA Mouse mAb (A10609)}

Western blot - NF-kB p65/RelA Mouse mAb (A10609)

Western blot analysis of various lysates using NF-kB p65/RelA Mouse mAb (A10609) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - NF-kB p65/RelA Mouse mAb (A10609)}

Immunofluorescence - NF-kB p65/RelA Mouse mAb (A10609)

Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Mouse mAb (A10609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NF-kB p65/RelA Mouse mAb (A10609)}

Immunofluorescence - NF-kB p65/RelA Mouse mAb (A10609)

Immunofluorescence analysis of C6 cells using NF-kB p65/RelA Mouse mAb (A10609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NF-kB p65/RelA Mouse mAb (A10609)}

Immunofluorescence - NF-kB p65/RelA Mouse mAb (A10609)

Immunofluorescence analysis of U2OS cells using NF-kB p65/RelA Mouse mAb (A10609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on RELA. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to RELA. (Distance between topics and target gene indicate popularity.) RELA

* Data provided by citexs.com, for reference only.

Publishing research using A10609? Please let us know so that we can cite the reference in this datasheet.

Antibodies (22)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (17)

* For research use only. Not for therapeutic or diagnostic purposes.

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