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NF-kB p65/RelA Mouse mAb (A10609)

Reviews (3)Publications (10) Datasheet

ABclonal:Western blot - NF-kB p65/RelA Mouse mAb (A10609)

Western blot analysis of extracts of various cell lines, using NF-kB p65 antibody (A10609) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Review
ABclonal: review for NF-kB p65/RelA Mouse mAb(A10609)
Review
ABclonal: review for NF-kB p65/RelA Mouse mAb(A10609)
Review
ABclonal: review for NF-kB p65/RelA Mouse mAb(A10609)

Overview

Product nameNF-kB p65/RelA Mouse mAb
Catalog No.A10609
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant protein of human NF-kB p65
SequenceEmail for sequence
Gene ID5970
Swiss protQ04206
SynonymsNFKB3; p65; NF-kB p65; RELA; CMCU
Calculated MW58kDa/59kDa/60kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, Jurkat, HT-29, SGC-7901, NIH/3T3, Mouse liver
Cellular locationCytoplasm, Nucleus
Customer validation

(Mononuclear macrophages, human cells)

WB(Sus scrofa, Mus musculus, Edwardsiella tarda, Mauremys sinensis , Cyprinus carpio, Homo sapiens)

IHC(Sus scrofa)

IF(Mus musculus)

IHC(Rattus norvegicus)

ABclonal:Western blot - NF-kB p65/RelA Mouse mAb (A10609) }

Western blot - NF-kB p65/RelA Mouse mAb (A10609)

Western blot analysis of extracts of various cell lines, using NF-kB p65 antibody (A10609) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

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