Products > Catalog Antibodies > Polyclonal Antibodies

NF-kB p65 Rabbit pAb (A2547)

Reviews (6)Publications (34) Datasheet

ABclonal:Western blot - NF-kB p65 Rabbit pAb (A2547)
ABclonal:Immunohistochemistry - NF-kB p65 Rabbit pAb (A2547)
ABclonal:Immunofluorescence - NF-kB p65 Rabbit pAb (A2547)
ABclonal:Immunofluorescence - NF-kB p65 Rabbit pAb (A2547)
ABclonal:Immunofluorescence - NF-kB p65 Rabbit pAb (A2547)
Review
ABclonal: review for NF-kB p65 Rabbit pAb(A2547)
Review
ABclonal: review for NF-kB p65 Rabbit pAb(A2547)
Review
ABclonal: review for NF-kB p65 Rabbit pAb(A2547)
Review
ABclonal: review for NF-kB p65 Rabbit pAb(A2547)
Review
ABclonal: review for NF-kB p65 Rabbit pAb(A2547)
Review
ABclonal: review for NF-kB p65 Rabbit pAb(A2547)

Overview

Product nameNF-kB p65 Rabbit pAb
Catalog No.A2547
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 50-180 of human NF-kB p65 (NP_068810.3).
SequenceRSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLS
Gene ID5970
Swiss protQ04206
SynonymsNFKB3; p65; NF-kB p65; RELA; CMCU
Calculated MW58kDa/59kDa/60kDa
Observed MW65KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, 293T, MCF7, Mouse lung, Mouse kidney, Rat lung, Rat kidney
Cellular locationCytoplasm, Nucleus
Customer validation

(Mouse atherosclerosis arterial tissue, RAW264.7, HCC827, U251, Rat H9c2 cells)

WB(Homo sapiens, Rattus norvegicus, Gallus gallus, Mus musculus, Macaca mulatta, Alpinia katsumadai Hayata, Sus scrofa, Schisandra chinensis)

ChIP(Homo sapiens)

IF(Mus musculus, Rattus norvegicus, Homo sapiens)

IHC(Mus musculus, Rattus norvegicus)

ABclonal:Western blot - NF-kB p65 Rabbit pAb (A2547) }

Western blot - NF-kB p65 Rabbit pAb (A2547)

Western blot analysis of extracts of various cell lines, using NF-kB p65 Rabbit pAb (A2547) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - NF-kB p65 Rabbit pAb (A2547) }

Immunohistochemistry - NF-kB p65 Rabbit pAb (A2547)

Immunohistochemistry of paraffin-embedded human stomach using NF-kB p65 Antibody (A2547) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - NF-kB p65 Rabbit pAb (A2547) }

Immunofluorescence - NF-kB p65 Rabbit pAb (A2547)

Immunofluorescence analysis of C6 cells using NF-kB p65 antibody (A2547) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NF-kB p65 Rabbit pAb (A2547) }

Immunofluorescence - NF-kB p65 Rabbit pAb (A2547)

Immunofluorescence analysis of L929 cells using NF-kB p65 antibody (A2547) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NF-kB p65 Rabbit pAb (A2547) }

Immunofluorescence - NF-kB p65 Rabbit pAb (A2547)

Immunofluorescence analysis of U2OS cells using NF-kB p65 antibody (A2547) at dilution of 1:100. Blue: DAPI for nuclear staining.

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