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NFKBIA Polyclonal Antibody (A1187)

Reviews (4)Publications (7) Datasheet

ABclonal:Western blot - NFKBIA antibody (A1187)

Overview

Product nameNFKBIA Polyclonal Antibody
Catalog No.A1187
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human NFKBIA (NP_065390.1).
SequenceMFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL
Gene ID4792
Swiss protP25963
SynonymsIKBA; MAD-3; NFKBI
Calculated MW35kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesDU145, Mouse thymus, Rat thymus
Cellular locationCytoplasm, Nucleus
Customer validation

IHC(Human glioma)

WB(Hela, HepG2, Mouse splenocytes, Human)

IF(Human)

IF(Mouse)

ABclonal:Western blot - NFKBIA antibody (A1187)}

Western blot - NFKBIA antibody (A1187)

Western blot analysis of extracts of various cell lines, using NFKBIA antibody (A1187) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

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