Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

NFKB1 Rabbit mAb (A11892)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - NFKB1 Rabbit mAb (A11892)

Western blot analysis of extracts of various cell lines, using NFKB1 antibody (A11892).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name NFKB1 Rabbit mAb
Catalog No. A11892
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. NFKB is a critical regulator of the immediate-early response to viral infection. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.

Immunogen information

Immunogen Recombinant protein of human NFKB1
Sequence Email for sequence
Gene ID 4790
Swiss prot P19838
Synonyms KBF1; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1; NF-kappabeta; NFKB1
Calculated MW 105kDa
Observed MW 47kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
  • IP 1:20 - 1:50
  • FC 1:20 - 1:50
  • ChIP 1:20 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Immunoprecipitation    Flow Cytometry    
Positive samples A20, BaF3, THP-1, Raji, NBT-II, Rat spleen
Cellular location Cytoplasm, Nucleus

Research Area

NFKB1 Rabbit mAb images

ABclonal:Western blot - NFKB1 Rabbit mAb (A11892)}

Western blot - NFKB1 Rabbit mAb (A11892)

Western blot analysis of extracts of various cell lines, using NFKB1 antibody (A11892).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on NFKB1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NFKB1. (Distance between topics and target gene indicate popularity.) NFKB1

* Data provided by citexs.com, for reference only.

Publishing research using A11892? Please let us know so that we can cite the reference in this datasheet.

Antibodies (10)

ELISA Kits (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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