Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

NFAT2 Rabbit pAb (A1539)

Publications (35) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - NFAT2 Rabbit pAb (A1539)

Western blot analysis of lysates from Mouse thymus, using NFAT2 Rabbit pAb (A1539) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - NFAT2 Rabbit pAb (A1539)

Immunohistochemistry analysis of NFAT2 in paraffin-embedded human placenta using NFAT2 Rabbit pAb (A1539) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - NFAT2 Rabbit pAb (A1539)

Immunofluorescence analysis of Jurkat cells using NFAT2 Rabbit pAb (A1539) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name NFAT2 Rabbit pAb
Catalog No. A1539
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of NFAT2 (NP_765978.1).
Sequence VFGRGETLGPAPRAGGTMKSAEEEHYGYASSNVSPALPLPTAHSTLPAPCHNLQTSTPGIIPPADHPSGYGAALDGGPAGYFLSSGHTRPDGAPALESPRIEITSCLGLYHNNNQFFHDVEVEDVLPSSKRSPSTATLSLPSLEAYRDPSCLSPASSLSSRSCNSEASSYESNYSYPYASPQTSPWQSPCVSPKTTDPEEGFPRGLGACTLLGSPRHSPSTSPRASVTEESWLGARSSRPASPCNKRKYSLNGRQPPYSPHHSPTPSPHGSPRVSVTDDSW
Gene ID 4772
Swiss prot O95644
Synonyms NFAT2; NFATc; NF-ATC; NF-ATc1.2
Calculated MW 101kDa
Observed MW 101kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Mouse thymus
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Mus musculus, Rattus norvegicus, Homo sapiens)

IHC (Mus musculus, Homo sapiens)

IF (Mus musculus, Rattus norvegicus, Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

NFAT2 Rabbit pAb images

ABclonal:Western blot - NFAT2 Rabbit pAb (A1539)}

Western blot - NFAT2 Rabbit pAb (A1539)

Western blot analysis of lysates from Mouse thymus, using NFAT2 Rabbit pAb (A1539) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - NFAT2 Rabbit pAb (A1539)}

Immunohistochemistry - NFAT2 Rabbit pAb (A1539)

Immunohistochemistry analysis of NFAT2 in paraffin-embedded human placenta using NFAT2 Rabbit pAb (A1539) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - NFAT2 Rabbit pAb (A1539)}

Immunofluorescence - NFAT2 Rabbit pAb (A1539)

Immunofluorescence analysis of Jurkat cells using NFAT2 Rabbit pAb (A1539) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on NFATC1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NFATC1. (Distance between topics and target gene indicate popularity.) NFATC1

* Data provided by citexs.com, for reference only.

Publishing research using A1539? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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