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N-Cadherin Rabbit pAb (A0433)

Review (1)Publications (10) Datasheet

ABclonal:Western blot - N-Cadherin Rabbit pAb (A0433)

Western blot analysis of extracts of various cell lines, using N-Cadherin antibody (A0433) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - N-Cadherin Rabbit pAb (A0433)

Immunohistochemistry of paraffin-embedded mouse liver using N-Cadherin Antibody (A0433) at dilution of 1:100 (40x lens).

Review
ABclonal: review for N-Cadherin Rabbit pAb(A0433)

Overview

Product nameN-Cadherin Rabbit pAb
Catalog No.A0433
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 750-850 of human N-Cadherin (NP_001783.2).
SequenceDKERQAKQLLIDPEDDVRDNILKYDEEGGGEEDQDYDLSQLQQPDTVEPDAIKPVGIRRMDERPIHAEPQYPVRSAAPHPGDIGDFINEGLKAADNDPTAP
Gene ID1000
Swiss protP19022
SynonymsCD325; CDHN; CDw325; NCAD; N Cadherin; CDH2
Calculated MW97kDa/99kDa
Observed MW140kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples293T, Mouse liver, Mouse brain, Mouse heart, Rat liver
Cellular locationCell membrane, Single-pass type I membrane protein
Customer validation

(U87MG)

IHC(Mus musculus)

WB(Homo sapiens, Mus musculus)

ICC(Homo sapiens)

IHC(Homo sapiens)

ABclonal:Western blot - N-Cadherin Rabbit pAb (A0433) }

Western blot - N-Cadherin Rabbit pAb (A0433)

Western blot analysis of extracts of various cell lines, using N-Cadherin antibody (A0433) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - N-Cadherin Rabbit pAb (A0433) }

Immunohistochemistry - N-Cadherin Rabbit pAb (A0433)

Immunohistochemistry of paraffin-embedded mouse liver using N-Cadherin Antibody (A0433) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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