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NCAM1 / CD56 Rabbit pAb (A7913)

Reviews (2)Publications (3) Datasheet

ABclonal:Western blot - NCAM1 / CD56 Rabbit pAb (A7913)
ABclonal:Immunohistochemistry - NCAM1 / CD56 Rabbit pAb (A7913)
ABclonal:Immunohistochemistry - NCAM1 / CD56 Rabbit pAb (A7913)
ABclonal:Immunofluorescence - NCAM1 / CD56 Rabbit pAb (A7913)
Review
ABclonal: review for NCAM1 / CD56 Rabbit pAb(A7913)
Review
ABclonal: review for NCAM1 / CD56 Rabbit pAb(A7913)

Overview

Product nameNCAM1 / CD56 Rabbit pAb
Catalog No.A7913
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 759-858 of human NCAM1 / CD56 (NP_851996.2).
SequenceLCGKAGPGAKGKDMEEGKAAFSKDESKEPIVEVRTEEERTPNHDGGKHTEPNETTPLTEPEKGPVEAKPECQETETKPAPAEVKTVPNDATQTKENESKA
Gene ID4684
Swiss protP13591
SynonymsCD56; MSK39; NCAM; NCAM1; NCAM1 /CD56
Calculated MW40kDa/73kDa/80kDa/83kDa/93kDa/94kDa
Observed MW160kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesMouse brain, Mouse spinal cord, Rat brain
Cellular locationCell membrane, GPI-anchor, Lipid-anchor, Secreted, Single-pass type I membrane protein
Customer validation

(mouse brain, Rat hippocampus)

WB(Homo sapiens)

IHC(Mus musculus)

ABclonal:Western blot - NCAM1 / CD56 Rabbit pAb (A7913) }

Western blot - NCAM1 / CD56 Rabbit pAb (A7913)

Western blot analysis of extracts of various cell lines, using NCAM1 / CD56 antibody (A7913) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - NCAM1 / CD56 Rabbit pAb (A7913) }

Immunohistochemistry - NCAM1 / CD56 Rabbit pAb (A7913)

Immunohistochemistry of paraffin-embedded mouse brain using NCAM1 / CD56 Rabbit pAb (A7913) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - NCAM1 / CD56 Rabbit pAb (A7913) }

Immunohistochemistry - NCAM1 / CD56 Rabbit pAb (A7913)

Immunohistochemistry of paraffin-embedded rat brain using NCAM1 / CD56 Rabbit pAb (A7913) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - NCAM1 / CD56 Rabbit pAb (A7913) }

Immunofluorescence - NCAM1 / CD56 Rabbit pAb (A7913)

Immunofluorescence analysis of C6 cells using NCAM1 / CD56 antibody (A7913) at dilution of 1:100. Blue: DAPI for nuclear staining.

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