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NBR1 Rabbit pAb (A3949)

Datasheet

ABclonal:Western blot - NBR1 Rabbit pAb (A3949)
ABclonal:Immunohistochemistry - NBR1 Rabbit pAb (A3949)
ABclonal:Immunoprecipitation - NBR1 Rabbit pAb (A3949)

Overview

Product nameNBR1 Rabbit pAb
Catalog No.A3949
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene was originally identified as an ovarian tumor antigen monitored in ovarian cancer. The encoded protein contains a B-box/coiled-coil motif, which is present in many genes with transformation potential. It functions as a specific autophagy receptor for the selective autophagic degradation of peroxisomes by forming intracellular inclusions with ubiquitylated autophagic substrates. This gene is located on a region of chromosome 17q21.1 that is in close proximity to the BRCA1 tumor suppressor gene. Alternative splicing of this gene results in multiple transcript variants.
ImmunogenRecombinant protein of human NBR1
SequenceEmail for sequence
Gene ID4077
Swiss protQ14596
SynonymsNBR1; 1A1-3B; IAI3B; M17S2; MIG19
Calculated MW103kDa/107kDa
Observed MW120KDa
ReactivityHuman, Mouse, Rat, Monkey
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesMouse testis
Cellular locationCytoplasm, Cytoplasmic vesicle, Lysosome, M line, autophagosome, myofibril, sarcomere
ABclonal:Western blot - NBR1 Rabbit pAb (A3949) }

Western blot - NBR1 Rabbit pAb (A3949)

Western blot analysis of extracts of Mouse testis, using NBR1 Rabbit pAb (A3949) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - NBR1 Rabbit pAb (A3949) }

Immunohistochemistry - NBR1 Rabbit pAb (A3949)

Immunohistochemistry of paraffin-embedded human liver, using NBR1 antibody (A3949).
ABclonal:Immunoprecipitation - NBR1 Rabbit pAb (A3949) }

Immunoprecipitation - NBR1 Rabbit pAb (A3949)

Immunoprecipitation analysis of extracts of K562 cells, using NBR1 antibody (A3949).

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