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NAP1L1 Rabbit pAb (A11610)

Datasheet

ABclonal:Western blot - NAP1L1 Polyclonal Antibody (A11610)

Overview

Product nameNAP1L1 Rabbit pAb
Catalog No.A11610
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the nucleosome assembly protein (NAP) family. This protein participates in DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms; however, not all have been fully described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human NAP1L1 (NP_004528.1).
SequenceMADIDNKEQSELDQDLDDVEEVEEEETGEETKLKARQLTVQMMQNPQILAALQERLDGLVETPTGYIESLPRVVKRRVNALKNLQVKCAQIEAKFYEEVHDLERKYAVLYQPLFDKRFEIINAIYEPTEEECEWKPDEEDEISEELKEKAKIEDEKKDEEKEDPKGIPEF
Gene ID4673
Swiss protP55209
SynonymsNAP1L1; NAP1; NAP1L; NRP
Calculated MW37kDa/42kDa/45kDa
Observed MW57kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, A375, LO2
Cellular locationMelanosome, Nucleus
ABclonal:Western blot - NAP1L1 Polyclonal Antibody (A11610) }

Western blot - NAP1L1 Polyclonal Antibody (A11610)

Western blot analysis of extracts of various cell lines, using NAP1L1 Antibody (A11610) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

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