Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Myelin Basic Protein Rabbit mAb (A11162)

Publications (3) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Mouse, Rat

ABclonal:Western blot - Myelin Basic Protein Rabbit mAb (A11162)

Western blot analysis of extracts of various cell lines, using Myelin Basic Protein Rabbit mAb (A11162) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Myelin Basic Protein Rabbit mAb (A11162)

Immunofluorescence analysis of rat brain using Myelin Basic Protein Rabbit mAb (A11162) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Myelin Basic Protein Rabbit mAb (A11162)

Immunofluorescence analysis of mouse brain using Myelin Basic Protein Rabbit mAb (A11162) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameMyelin Basic Protein Rabbit mAb
Catalog No.A11162
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0535

Background

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]

Immunogen information

ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 161-304 of human Myelin Basic Protein (P02686).
SequenceGFLPRHRDTGILDSIGRFFGGDRGAPKRGSGKDSHHPARTAHYGSLPQKSHGRTQDENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQRPGFGYGGRASDYKSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSPMARR
Gene ID4155
Swiss protP02686
SynonymsMBP; myelin basic protein
Calculated MW18kDa
Observed MW14-23KDa

Applications

ReactivityMouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMouse brain, Rat brain
Cellular locationCytoplasmic side, Myelin membrane, Nucleus, Peripheral membrane protein
Customer validation

WB(Mus musculus, Arabidopsis thaliana, Rattus norvegicus)

IHC(Rattus norvegicus)

Research Area

Myelin Basic Protein Rabbit mAb images

ABclonal:Western blot - Myelin Basic Protein Rabbit mAb (A11162)}

Western blot - Myelin Basic Protein Rabbit mAb (A11162)

Western blot analysis of extracts of various cell lines, using Myelin Basic Protein Rabbit mAb (A11162) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - Myelin Basic Protein Rabbit mAb (A11162)}

Immunofluorescence - Myelin Basic Protein Rabbit mAb (A11162)

Immunofluorescence analysis of rat brain using Myelin Basic Protein Rabbit mAb (A11162) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Myelin Basic Protein Rabbit mAb (A11162)}

Immunofluorescence - Myelin Basic Protein Rabbit mAb (A11162)

Immunofluorescence analysis of mouse brain using Myelin Basic Protein Rabbit mAb (A11162) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on MBP. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MBP. (Distance between topics and target gene indicate popularity.) MBP

* Data provided by citexs.com, for reference only.

Publishing research using A11162? Please let us know so that we can cite the reference in this datasheet.

Primary Antibodies (1)

ELISA Kits (1)

Secondary Antibodies (26)

* For research use only. Not for therapeutic or diagnostic purposes.

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