Product Type > Antibodies > Tag and Loading Control Antibodies

Mouse anti TAP-Tag mAb (AE021)

Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:

ABclonal:Western blot - Mouse anti TAP-Tag mAb (AE021)

Western blot analysis of recombinant TAP protein using mouse anti TAP-Tag mAb (AE021) at dilution of 1:5000.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Overview

Product nameMouse anti TAP-Tag mAb
Catalog No.AE021
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1
CloneNo.AMC0510

Background

Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

Immunogen information

ImmunogenA synthetic peptide of TAP-Tag
SequenceCSSGALDYDIPTTASENLYFQ
Gene ID
Swiss prot
SynonymsTAP; TAP tag; TAP-tag
Calculated MW
Observed MWRefer to Figures

Applications

Reactivity
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:5000 - 1:10000
  • IP 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samples
Cellular location

Mouse anti TAP-Tag mAb images

ABclonal:Western blot - Mouse anti TAP-Tag mAb (AE021)}

Western blot - Mouse anti TAP-Tag mAb (AE021)

Western blot analysis of recombinant TAP protein using mouse anti TAP-Tag mAb (AE021) at dilution of 1:5000.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on TAP. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TAP. (Distance between topics and target gene indicate popularity.) TAP

* Data provided by citexs.com, for reference only.

Publishing research using AE021? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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