Products > Catalog Antibodies > Tag and Loading Control Antibodies

Mouse anti His-Tag mAb (AE003)

Publications (13) Datasheet

ABclonal:Western blot - His-tag antibody (AE003)
ABclonal:Immunofluorescence - Mouse anti His-Tag mAb (AE003)
ABclonal:Immunoprecipitation - His-tag antibody (AE003)

Overview

Product nameMouse anti His-Tag mAb
Catalog No.AE003
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
ImmunogenA synthetic peptide of His tag
SequenceEmail for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:2000 - 1:5000
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples293T
Cellular location
Customer validation

WB(unknown, Rabbit, Human, Oyster, Cyprinus carpio, G.xylinus, Plant, Crabs)

IF(unknown)

ABclonal:Western blot - His-tag antibody (AE003)}

Western blot - His-tag antibody (AE003)

Western blot analysis of over-expressed His-tagged protein in 293T cell using His-tag antibody (AE003) at different dilution. Each lane was loaded with 2 ug cell lysate.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - Mouse anti His-Tag mAb (AE003)}

Immunofluorescence - Mouse anti His-Tag mAb (AE003)

Immunofluorescence analysis of 293T cells using Mouse anti His-Tag mAb (AE003). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - His-tag antibody (AE003)}

Immunoprecipitation - His-tag antibody (AE003)

Immunoprecipitation of over-expressed His-tagged protein in 293T cells incubated using His-tag antibody (AE003). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

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