Catalog No:AE003
Host species:Mouse
Purity:Affinity purification
ABclonal:Western blot - His-tag antibody (AE003)
ABclonal:Immunofluorescence - Mouse anti His-Tag mAb (AE003)
ABclonal:Immunoprecipitation - His-tag antibody (AE003)

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Immunogen information

ImmunogenA synthetic peptide of His tag
SequenceEmail for sequence
Gene ID
Swiss Prot
Calculated MW
Observed MW


Tested applicationsWB, IF, IP
Recommended DilutionWB 1:2000 - 1:5000
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples293T
Cellular Location
Customer Validation

WB (Human, Oyster, Rabbit, Cyprinus carpio, Plant, G.xylinus, Crabs)

IF ()

Mouse anti His-Tag mAb images

ABclonal:Western blot - His-tag antibody (AE003)

Western blot - His-tag antibody (AE003)

Western blot analysis of over-expressed His-tagged protein in 293T cell using His-tag antibody (AE003) at different dilution. Each lane was loaded with 2 ug cell lysate.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - Mouse anti His-Tag mAb (AE003)

Immunofluorescence - Mouse anti His-Tag mAb (AE003)

Immunofluorescence analysis of 293T cells using Mouse anti His-Tag mAb (AE003). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - His-tag antibody (AE003)

Immunoprecipitation - His-tag antibody (AE003)

Immunoprecipitation of over-expressed His-tagged protein in 293T cells incubated using His-tag antibody (AE003). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

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