Product Type > Antibodies > Tag and Loading Control Antibodies

Mouse anti DDDDK-Tag mAb (AE005)

Publications (201) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Species independent

ABclonal:Western blot - Mouse anti DDDDK-Tag mAb (AE005)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with Renin using Mouse anti DDDDK-Tag mAb(AE005) at 1:5000 dilution. 
Secondary antibody:HRP Goat Anti-Mouse IgG (H+L)(AS003) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - Mouse anti DDDDK-Tag mAb (AE005)

Immunofluorescence analysis of 293T cells using Mouse anti DDDDK-Tag mAb (AE005) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Mouse anti DDDDK-Tag mAb (AE005)

Immunofluorescence analysis of 293T-BCAT2-Flag-GFP-C cells using Mouse anti DDDDK-Tag mAb (AE005) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Mouse anti DDDDK-Tag mAb (AE005)

Immunofluorescence analysis of 293T-BCAT2-Flag-GFP-N cells using Mouse anti DDDDK-Tag mAb (AE005) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Mouse anti DDDDK-Tag mAb (AE005)

Immunoprecipitation analysis of 200ug extract cell lysate from 293T cells transfected with GSK3B expression vector containing a DDDDK-Tag with 3 μg Mouse anti DDDDK-Tag mAb antibody(AE005).Western blot was performed from the immunoprecipitate using Mouse anti DDDDK-Tag mAb antibody(AE005).

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Overview

Product name Mouse anti DDDDK-Tag mAb
Catalog No. AE005
Host species Mouse
Purification method Affinity purification
Isotype IgG1, Kappa
CloneNo. AMC0382

Background

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.

Immunogen information

Immunogen A synthetic peptide corresponding to DDDDK tag.
Sequence DYKDDDDK
Gene ID
Swiss prot
Synonyms DDDDK; DDDDK tag; DDDDK-tag
Calculated MW
Observed MW 55kDa/48kDa/40kDa

Applications

Reactivity Species independent
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Immunoprecipitation    
Positive samples 293T-GSK3B-Flag(C-terminal), 293T-IRF1-Flag(3*N-terminal), 293T
Cellular location
Customer validation

WB (Homo sapiens, Oryza sativa, Arabidopsis thaliana, Mus musculus, Sus scrofa, Drosophila melanogaster, Ctenopharyngodon idellus, Escherichia coli, Cyprinus carpio, Grapevine, Lycopersicon esculentum, G.hirsutum, Phytophthora capsici, N. benthamiana, Pimephales promelas, Nicotiana benthamiana, Cherax quadricarinatus, Danio rerio, Zea mays, Oryctolagus cuniculus, Triticum aestivum, Cyprinus Carpio, Bacillus altitudinis, Nepenthes, D. melanogaste, Xenopus laevis, Rattus norvegicus, Saccharomyces cerevisiae, Caenorhabditis elegans, wide range, Ictalurus punctatus, Stemphylium eturmiunum, Capra hircus)

Co-IP (Oryza sativa, Toxoplasma gondii, Homo sapiens, PnianaPinus massoniana, Danio rerio, Oryza sativa L., Chlorocebus pygerythrus, Xenopus laevis, Caenorhabditis elegans, Mus musculus, Stemphylium eturmiunum, B. napus)

IF (Danio rerio, Homo sapiens, Gallus gallus, Paralichthys olivaceus, Bos taurus, Rattus norvegicus, Mus musculus, Escherichia coli, D. melanogaste, Sus scrofa)

IP (Homo sapiens, Yeast, Danio rerio, Nicotiana benthamiana, Mus musculus, Helicoverpa armigera, Drosophila melanogaster, Nepenthes, Sus scrofa, O. sativa L., Ictalurus punctatus)

RIP (Homo sapiens, Drosophila melanogaster)

co-ip (Homo sapiens)

IB (Homo sapiens)

HE (Mus musculus, Homo sapiens)

ChIP (Mus musculus, Drosophila melanogaster, Other)

ELISA (Mus musculus)

IHC (Homo sapiens)

ChIP-seq (Saccharomyces cerevisiae)

CHIP (Arabidopsis thaliana)

Documents

Certificate of Compliance

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Lot number

Mouse anti DDDDK-Tag mAb images

ABclonal:Western blot - Mouse anti DDDDK-Tag mAb (AE005)}

Western blot - Mouse anti DDDDK-Tag mAb (AE005)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with Renin using Mouse anti DDDDK-Tag mAb(AE005) at 1:5000 dilution. 
Secondary antibody:HRP Goat Anti-Mouse IgG (H+L)(AS003) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - Mouse anti DDDDK-Tag mAb (AE005)}

Immunofluorescence - Mouse anti DDDDK-Tag mAb (AE005)

Immunofluorescence analysis of 293T cells using Mouse anti DDDDK-Tag mAb (AE005) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Mouse anti DDDDK-Tag mAb (AE005)}

Immunofluorescence - Mouse anti DDDDK-Tag mAb (AE005)

Immunofluorescence analysis of 293T-BCAT2-Flag-GFP-C cells using Mouse anti DDDDK-Tag mAb (AE005) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Mouse anti DDDDK-Tag mAb (AE005)}

Immunofluorescence - Mouse anti DDDDK-Tag mAb (AE005)

Immunofluorescence analysis of 293T-BCAT2-Flag-GFP-N cells using Mouse anti DDDDK-Tag mAb (AE005) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Mouse anti DDDDK-Tag mAb (AE005)}

Immunoprecipitation - Mouse anti DDDDK-Tag mAb (AE005)

Immunoprecipitation analysis of 200ug extract cell lysate from 293T cells transfected with GSK3B expression vector containing a DDDDK-Tag with 3 μg Mouse anti DDDDK-Tag mAb antibody(AE005).Western blot was performed from the immunoprecipitate using Mouse anti DDDDK-Tag mAb antibody(AE005).

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Related Targets

Based on existing publications, the following genes are closely related to research on DDDDK. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to DDDDK. (Distance between topics and target gene indicate popularity.) DDDDK

* Data provided by citexs.com, for reference only.

Publishing research using AE005? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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