Product Type > Antibodies > Primary Antibodies > Methyl-specific Antibodies

MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)

Western blot analysis of various lysates using MonoMethyl-Histone H4-R3 Rabbit pAb (A3158).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)

Immunohistochemistry analysis of MonoMethyl-Histone H4-R3 in paraffin-embedded rat lung using MonoMethyl-Histone H4-R3 Rabbit pAb (A3158) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)

Immunohistochemistry analysis of MonoMethyl-Histone H4-R3 in paraffin-embedded rat brain using MonoMethyl-Histone H4-R3 Rabbit pAb (A3158) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)

Immunohistochemistry analysis of MonoMethyl-Histone H4-R3 in paraffin-embedded human breast cancer using MonoMethyl-Histone H4-R3 Rabbit pAb (A3158) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)

Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-R3 Rabbit pAb (A3158). Blue: DAPI for nuclear staining.

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Overview

Product name MonoMethyl-Histone H4-R3 Rabbit pAb
Catalog No. A3158
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Immunogen information

Immunogen A synthetic monomethylated peptide around R3 of human Histone H4 (NP_003529.1).
Sequence SGRGK
Gene ID 8359
Swiss prot P62805
Synonyms H4/I; H4C1; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4FI; H4-16; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; HIST1H4B; MonoMethyl-Histone H4-R3
Calculated MW 11kDa
Observed MW 13kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa
Cellular location Chromosome, Nucleus

Documents

Certificate of Compliance

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Research Area

MonoMethyl-Histone H4-R3 Rabbit pAb images

ABclonal:Western blot - MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)}

Western blot - MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)

Western blot analysis of various lysates using MonoMethyl-Histone H4-R3 Rabbit pAb (A3158).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)}

Immunohistochemistry - MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)

Immunohistochemistry analysis of MonoMethyl-Histone H4-R3 in paraffin-embedded rat lung using MonoMethyl-Histone H4-R3 Rabbit pAb (A3158) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)}

Immunohistochemistry - MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)

Immunohistochemistry analysis of MonoMethyl-Histone H4-R3 in paraffin-embedded rat brain using MonoMethyl-Histone H4-R3 Rabbit pAb (A3158) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)}

Immunohistochemistry - MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)

Immunohistochemistry analysis of MonoMethyl-Histone H4-R3 in paraffin-embedded human breast cancer using MonoMethyl-Histone H4-R3 Rabbit pAb (A3158) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)}

Immunofluorescence - MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)

Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-R3 Rabbit pAb (A3158). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on H4C2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H4C2. (Distance between topics and target gene indicate popularity.) H4C2

* Data provided by citexs.com, for reference only.

Publishing research using A3158? Please let us know so that we can cite the reference in this datasheet.

Antibodies (33)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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