Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat, Other (Wide Range)
Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-R8 antibody (A3156) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-R8 antibody (A3156) at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat heart using MonoMethyl-Histone H3-R8 antibody (A3156) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human breast cancer using MonoMethyl-Histone H3-R8 antibody (A3156) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse testis using MonoMethyl-Histone H3-R8 antibody (A3156) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
| Product name | MonoMethyl-Histone H3-R8 Rabbit pAb |
|---|---|
| Catalog No. | A3156 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Reactivity | Human, Mouse, Rat, Other (Wide Range) |
|---|---|
| Tested applications | WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us. |
| Application key | Western blotting Immunohistochemistry Immunoprecipitation |
| Positive samples | HeLa, NIH/3T3, C6 |
| Cellular location | Chromosome, Nucleus |
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Based on existing publications, the following genes are closely related to research on Histone H3. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to Histone H3. (Distance between topics and target gene indicate popularity.) Histone H3
* Data provided by citexs.com, for reference only.
Publishing research using A3156? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.
