Product Type > Antibodies > Primary Antibodies > Methyl-specific Antibodies

MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Western blot analysis of lysates from C6 cells, using MonoMethyl-Histone H3-R17 Rabbit pAb (A3151) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Dot Blot - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-R17 antibody (A3151).

ABclonal:Immunohistochemistry - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Immunohistochemistry analysis of MonoMethyl-Histone H3-R17 in paraffin-embedded rat lung using MonoMethyl-Histone H3-R17 Rabbit pAb (A3151) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Immunohistochemistry analysis of MonoMethyl-Histone H3-R17 in paraffin-embedded human breast using MonoMethyl-Histone H3-R17 Rabbit pAb (A3151) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Immunohistochemistry analysis of MonoMethyl-Histone H3-R17 in paraffin-embedded mouse kidney using MonoMethyl-Histone H3-R17 Rabbit pAb (A3151) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Immunofluorescence analysis of C6 cells using MonoMethyl-Histone H3-R17 Rabbit pAb (A3151) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Immunofluorescence analysis of NIH-3T3 cells using MonoMethyl-Histone H3-R17 Rabbit pAb (A3151) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H3-R17 Rabbit pAb (A3151) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name MonoMethyl-Histone H3-R17 Rabbit pAb
Catalog No. A3151
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen A synthetic monomethylated peptide around R17 of human histone H3 (NP_003520.1).
Sequence APRKQ
Gene ID 82908350
Swiss prot Q16695P68431
Synonyms H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J; MonoMethyl-Histone H3-R17
Calculated MW 15kDa
Observed MW 17kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples C6
Cellular location Chromosome, Nucleus

Documents

Certificate of Compliance

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MonoMethyl-Histone H3-R17 Rabbit pAb images

ABclonal:Western blot - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)}

Western blot - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Western blot analysis of lysates from C6 cells, using MonoMethyl-Histone H3-R17 Rabbit pAb (A3151) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Dot Blot - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)}

Dot Blot - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-R17 antibody (A3151).
ABclonal:Immunohistochemistry - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)}

Immunohistochemistry - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Immunohistochemistry analysis of MonoMethyl-Histone H3-R17 in paraffin-embedded rat lung using MonoMethyl-Histone H3-R17 Rabbit pAb (A3151) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)}

Immunohistochemistry - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Immunohistochemistry analysis of MonoMethyl-Histone H3-R17 in paraffin-embedded human breast using MonoMethyl-Histone H3-R17 Rabbit pAb (A3151) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)}

Immunohistochemistry - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Immunohistochemistry analysis of MonoMethyl-Histone H3-R17 in paraffin-embedded mouse kidney using MonoMethyl-Histone H3-R17 Rabbit pAb (A3151) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)}

Immunofluorescence - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Immunofluorescence analysis of C6 cells using MonoMethyl-Histone H3-R17 Rabbit pAb (A3151) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)}

Immunofluorescence - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Immunofluorescence analysis of NIH-3T3 cells using MonoMethyl-Histone H3-R17 Rabbit pAb (A3151) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)}

Immunofluorescence - MonoMethyl-Histone H3-R17 Rabbit pAb (A3151)

Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H3-R17 Rabbit pAb (A3151) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on H3C12. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H3C12. (Distance between topics and target gene indicate popularity.) H3C12

* Data provided by citexs.com, for reference only.

Publishing research using A3151? Please let us know so that we can cite the reference in this datasheet.

Antibodies (114)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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