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MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)

Reviews (3)Publications (13) Datasheet

ABclonal:MonoMethyl-Histone H3-K4 Rabbit pAb
ABclonal:Western blot - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)

Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K4 antibody (A2355) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal: - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K4 antibody (A2355).

ABclonal:Immunohistochemistry - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)

Immunohistochemistry of paraffin-embedded rat spleen using MonoMethyl-Histone H3-K4 antibody (A2355) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)

Immunohistochemistry of paraffin-embedded human thyroid cancer using MonoMethyl-Histone H3-K4 antibody (A2355) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)

Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H3-K4 antibody (A2355) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K4 antibody (A2355) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review
ABclonal: review for MonoMethyl-Histone H3-K4 Rabbit pAb(A2355)
Review
ABclonal: review for MonoMethyl-Histone H3-K4 Rabbit pAb(A2355)
Review
ABclonal: review for MonoMethyl-Histone H3-K4 Rabbit pAb(A2355)

Overview

Product nameMonoMethyl-Histone H3-K4 Rabbit pAb
Catalog No.A2355
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3
SequenceEmail for sequence
Gene ID8290
Swiss protQ16695
SynonymsH3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Calculated MW15kDa
Observed MW17KDa
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
ChIP-seq 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, NIH/3T3, C6
Cellular locationChromosome, Nucleus
Customer validation

(Prawn gills, Human cells, HeLa)

WB(Mus musculus, Solanum lycopersicum, Homo sapiens)

ChIP(Homo sapiens, Oryza sativa, Mus musculus)

ChIP-Seq(Zea mays)

ABclonal:Western blot - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355) }

Western blot - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)

Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K4 antibody (A2355) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal: - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355) }

- MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K4 antibody (A2355).
ABclonal:Immunohistochemistry - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355) }

Immunohistochemistry - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)

Immunohistochemistry of paraffin-embedded rat spleen using MonoMethyl-Histone H3-K4 antibody (A2355) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355) }

Immunohistochemistry - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)

Immunohistochemistry of paraffin-embedded human thyroid cancer using MonoMethyl-Histone H3-K4 antibody (A2355) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355) }

Immunofluorescence - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)

Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H3-K4 antibody (A2355) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355) }

Chromatin Immunoprecipitation - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K4 antibody (A2355) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

* For research use only. Not for therapeutic or diagnostic purposes.

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