Reviews (2)Publications (6) Datasheet SDS
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range)
Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K36 antibody (A2364) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K36 antibody (A2364).
Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K36 antibody (A2364). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K36 antibody (A2364) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
| Product name | MonoMethyl-Histone H3-K36 Rabbit pAb |
|---|---|
| Catalog No. | A2364 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Reactivity | Human, Mouse, Rat, Other (Wide Range) |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us. |
| Application key | Western blotting Immunofluorescence Immunoprecipitation |
| Positive samples | HeLa, NIH/3T3, C6 |
| Cellular location | Chromosome, Nucleus |
| Customer validation | (HeLa, Hippocampus) WB(Homo sapiens, Mus musculus, Oryza sativa) |
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Based on existing publications, the following genes are closely related to research on H3-4. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to H3-4. (Distance between topics and target gene indicate popularity.) H3-4
* Data provided by citexs.com, for reference only.
Publishing research using A2364? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.
