Product Type > Antibodies > Primary Antibodies > Methyl-specific Antibodies

MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

Reviews (2)Publications (6) Datasheet SDS

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal:Western blot - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K36 antibody (A2364) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Dot Blot - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K36 antibody (A2364).

ABclonal:Immunofluorescence - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K36 antibody (A2364). Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K36 antibody (A2364) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review
ABclonal: review for MonoMethyl-Histone H3-K36 Rabbit pAb(A2364)
Review
ABclonal: review for MonoMethyl-Histone H3-K36 Rabbit pAb(A2364)

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    Overview

    Product nameMonoMethyl-Histone H3-K36 Rabbit pAb
    Catalog No.A2364
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG

    Background

    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

    Immunogen information

    ImmunogenA synthetic monomethylated peptide around K36 of human histone H3 (NP_003520.1).
    SequenceGVKKP
    Gene ID8290
    Swiss protQ16695
    SynonymsH3t; H3.4; H3/g; H3FT; H3C16; HIST3H3
    Calculated MW16kDa
    Observed MW17KDa

    Applications

    ReactivityHuman, Mouse, Rat, Other (Wide Range)
    Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition
    Recommended dilution
    • WB 1:500 - 1:1000
    • IF/ICC 1:50 - 1:200
    • IP 1:50 - 1:200
    • ChIP 1:20 - 1:100
    • ChIP-seq 1:20 - 1:100
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
    Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
    Positive samplesHeLa, NIH/3T3, C6
    Cellular locationChromosome, Nucleus
    Customer validation

    (HeLa, Hippocampus)

    WB(Homo sapiens, Mus musculus, Oryza sativa)

    Research Area

    MonoMethyl-Histone H3-K36 Rabbit pAb images

    ABclonal:Western blot - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)}

    Western blot - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

    Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K36 antibody (A2364) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Dot Blot - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)}

    Dot Blot - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

    Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K36 antibody (A2364).
    ABclonal:Immunofluorescence - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)}

    Immunofluorescence - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

    Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K36 antibody (A2364). Blue: DAPI for nuclear staining.
    ABclonal:Chromatin Immunoprecipitation - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)}

    Chromatin Immunoprecipitation - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

    Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K36 antibody (A2364) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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    Related Targets

    Based on existing publications, the following genes are closely related to research on H3-4. (Numbers indicate number of published articles where both targets appear.)

    1/

    Related Research Area

    Based on existing publications, the following research topics are related to H3-4. (Distance between topics and target gene indicate popularity.) H3-4

    * Data provided by citexs.com, for reference only.

    Publishing research using A2364? Please let us know so that we can cite the reference in this datasheet.

    Primary Antibodies (50)

    ELISA Kits (1)

    Secondary Antibodies (24)

    * For research use only. Not for therapeutic or diagnostic purposes.

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