Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K36 antibody (A2364) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K36 antibody (A2364).
Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K36 antibody (A2364). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K36 antibody (A2364) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
|Product name||MonoMethyl-Histone H3-K36 Rabbit pAb|
|Purification method||Affinity purification|
|Reactivity||Human, Mouse, Rat, Other (Wide Range)|
|Tested applications||WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.|
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting Immunofluorescence Immunoprecipitation|
|Positive samples||HeLa, NIH/3T3, C6|
|Cellular location||Chromosome, Nucleus|
WB(Homo sapiens, Mus musculus, Oryza sativa)
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* For research use only. Not for therapeutic or diagnostic purposes.