Products > Catalog Antibodies > Methyl-specific Antibodies

MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

Reviews (2)Publications (5) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal:Western blot - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K36 antibody (A2364) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal: - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K36 antibody (A2364).

ABclonal:Immunofluorescence - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K36 antibody (A2364). Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K36 antibody (A2364) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review
ABclonal: review for MonoMethyl-Histone H3-K36 Rabbit pAb(A2364)
Review
ABclonal: review for MonoMethyl-Histone H3-K36 Rabbit pAb(A2364)

Overview

Product nameMonoMethyl-Histone H3-K36 Rabbit pAb
Catalog No.A2364
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic monomethylated peptide around K36 of human histone H3 (NP_003520.1).
SequenceGVKKP
Gene ID8290
Swiss protQ16695
SynonymsH3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Calculated MW15kDa
Observed MW17KDa
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IF 1:50 - 1:200
  • IP 1:50 - 1:200
  • ChIP 1:20 - 1:100
  • ChIP-seq 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, NIH/3T3, C6
Cellular locationChromosome, Nucleus
Customer validation

(HeLa, Hippocampus)

WB(Homo sapiens, Mus musculus, Oryza sativa)

ABclonal:Western blot - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)}

Western blot - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K36 antibody (A2364) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal: - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)}

- MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K36 antibody (A2364).
ABclonal:Immunofluorescence - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)}

Immunofluorescence - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K36 antibody (A2364). Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)}

Chromatin Immunoprecipitation - MonoMethyl-Histone H3-K36 Rabbit pAb (A2364)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K36 antibody (A2364) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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