Product Type > Antibodies > Primary Antibodies > Methyl-specific Antibodies

MonoMethyl-H2B-E105 Rabbit pAb (A20230)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - MonoMethyl-H2B-E105 Rabbit pAb (A20230)

Western blot analysis of various lysates using MonoMethyl-H2B-E105 Rabbit pAb (A20230) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

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Overview

Product name MonoMethyl-H2B-E105 Rabbit pAb
Catalog No. A20230
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen A synthetic monomethylated peptide around E105 of human H2B (NP_066406.1).
Sequence LPGEL
Gene ID 3018
Swiss prot P33778
Synonyms H2B.1; H2B/f; H2BFF; HIST1H2BB; MonoMethyl-H2B-E105
Calculated MW 14kDa
Observed MW 15kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa, NIH/3T3
Cellular location cytosol, nucleoplasm, nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

MonoMethyl-H2B-E105 Rabbit pAb images

ABclonal:Western blot - MonoMethyl-H2B-E105 Rabbit pAb (A20230)}

Western blot - MonoMethyl-H2B-E105 Rabbit pAb (A20230)

Western blot analysis of various lysates using MonoMethyl-H2B-E105 Rabbit pAb (A20230) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

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Related Targets

Based on existing publications, the following genes are closely related to research on H2BC3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H2BC3. (Distance between topics and target gene indicate popularity.) H2BC3

* Data provided by citexs.com, for reference only.

Publishing research using A20230? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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