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Mitofusin 2 Polyclonal Antibody (A12771)

Review (2) Datasheet

ABclonal:Western blot - Mitofusin 2 Polyclonal Antibody (A12771)
ABclonal:Immunofluorescence - Mitofusin 2 Polyclonal Antibody (A12771)
Review
ABclonal: review for Mitofusin 2 Polyclonal Antibody(A12771)
Review
ABclonal: review for Mitofusin 2 Polyclonal Antibody(A12771)

Overview

Product nameMitofusin 2 Polyclonal Antibody
Catalog No.A12771
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 500-600 of human Mitofusin 2 (NP_001121132.1).
SequenceGLKPLLPVSVRSQIDMLVPRQCFSLNYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALMGYNDQVQRPIPLTPANPSMPPLPQGSLTQEEFM
Gene ID9927
Swiss protO95140
SynonymsMFN2; CMT2A; CMT2A2; CMT2A2A; CMT2A2B; CPRP1; HMSN6A; HSG; MARF; mitofusin-2
Calculated MW50kDa/86kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRaji, MCF7, 293T, mouse brain, rat brain
Cellular locationMitochondrion outer membrane, Multi-pass membrane protein
Customer validation

(, )

WB(Rat)

ABclonal:Western blot - Mitofusin 2 Polyclonal Antibody (A12771) }

Western blot - Mitofusin 2 Polyclonal Antibody (A12771)

Western blot analysis of extracts of various cell lines, using Mitofusin 2 antibody (A12771) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - Mitofusin 2 Polyclonal Antibody (A12771) }

Immunofluorescence - Mitofusin 2 Polyclonal Antibody (A12771)

Immunofluorescence analysis of U-2 OS cells using MFN2 antibody (A12771) at dilution of 1:100. Blue: DAPI for nuclear staining.

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