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Map2 Polyclonal Antibody (A3278)

Datasheet

ABclonal:Western blot - Map2 Polyclonal Antibody (A3278)

Overview

Product nameMap2 Polyclonal Antibody
Catalog No.A3278
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described.
ImmunogenA synthetic peptide of mouse Map2
SequenceEmail for sequence
Gene ID17756
Swiss prot
SynonymsMAP2; MAP2A; MAP2B; MAP2C; microtubule-associated protein 2
Calculated MW200kDa
Observed MW80kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesOVCAR3, 293T, HepG2, SH-SY5Y, Mouse brain, Mouse thymus
Cellular locationCytoplasm, cytoskeleton
ABclonal:Western blot - Map2 Polyclonal Antibody (A3278) }

Western blot - Map2 Polyclonal Antibody (A3278)

Western blot analysis of extracts of various cell lines, using Map2 antibody (A3278) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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