Product Type > Antibodies > Tag and Loading Control Antibodies

Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb (AE037)

Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Species independent

ABclonal:Immunoprecipitation - Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb (AE037)

Immunoprecipitation analysis of 300 ug extract cell lysate from 293T cells transfected with GSK3B expression vector containing a C-terminal DDDDK-Tag (1x) with 30uL Magnetic Beads-conjugated Mouse anti DDDDK-tag mAb antibody(AE037). Magnetic Beads-conjugated mouse IgG isotype control pAb (AC044N) was used as a negative control. Western blot was performed from the immunoprecipitate using Rabbit anti DDDDK-Tag mAb antibody (AE092) at 1:10000 dilution.

ABclonal:Immunoprecipitation - Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb (AE037)

Immunoprecipitation analysis of 600ug extract cell lysate from 293T transfected with SERPINB1 expression vector containing a N-terminal DDDDK-Tag (1x) with 30ul Magnetic Beads-conjugated Mouse anti DDDDK-Tag antibody (AE037). Magnetic Beads-conjugated mouse IgG isotype control pAb (AC044N) was used as a negative control. Western blot was performed from the immunoprecipitate using Rabbit anti DDDDK-Tag mAb antibody (AE092) at 1:5000 dilution.

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Overview

Product name Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb
Catalog No. AE037
Host species Mouse
Purification method Affinity purification
Isotype IgG1
CloneNo. AMC0515

Background

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.

Immunogen information

Immunogen A synthetic peptide corresponding to DDDDK tag.
Sequence DYKDDDDK
Gene ID
Swiss prot
Synonyms DDDDK; DDDDK tag; DDDDK-tag
Calculated MW
Observed MW 50kDa/48kDa

Applications

Reactivity Species independent
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • IP 30ul antibody (bead slurry) for 200μg-400μg extracts of whole cells
Storage buffer Store at 4℃ Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, pH7.3.
Application key Immunoprecipitation    
Positive samples 293T-SERPINB1-Flag (N-terminal) , 293T-GSK3B-Flag(C-terminal)
Cellular location
Customer validation

Co-IP (Cherax quadricarinatus, Nicotiana benthamiana)

Documents

Certificate of Compliance

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Lot number

Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb images

ABclonal:Immunoprecipitation - Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb (AE037)}

Immunoprecipitation - Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb (AE037)

Immunoprecipitation analysis of 300 ug extract cell lysate from 293T cells transfected with GSK3B expression vector containing a C-terminal DDDDK-Tag (1x) with 30uL Magnetic Beads-conjugated Mouse anti DDDDK-tag mAb antibody(AE037). Magnetic Beads-conjugated mouse IgG isotype control pAb (AC044N) was used as a negative control. Western blot was performed from the immunoprecipitate using Rabbit anti DDDDK-Tag mAb antibody (AE092) at 1:10000 dilution.
ABclonal:Immunoprecipitation - Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb (AE037)}

Immunoprecipitation - Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb (AE037)

Immunoprecipitation analysis of 600ug extract cell lysate from 293T transfected with SERPINB1 expression vector containing a N-terminal DDDDK-Tag (1x) with 30ul Magnetic Beads-conjugated Mouse anti DDDDK-Tag antibody (AE037). Magnetic Beads-conjugated mouse IgG isotype control pAb (AC044N) was used as a negative control. Western blot was performed from the immunoprecipitate using Rabbit anti DDDDK-Tag mAb antibody (AE092) at 1:5000 dilution.

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Related Targets

Based on existing publications, the following genes are closely related to research on DDDDK. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to DDDDK. (Distance between topics and target gene indicate popularity.) DDDDK

* Data provided by citexs.com, for reference only.

Publishing research using AE037? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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