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MYCN Polyclonal Antibody (A0499)

Review (1)Publication (2) Datasheet

ABclonal:Western blot - MYCN Polyclonal Antibody (A0499)
ABclonal:Immunohistochemistry - MYCN Polyclonal Antibody (A0499)
ABclonal:Immunohistochemistry - MYCN Polyclonal Antibody (A0499)
Review
ABclonal: review for MYCN Polyclonal Antibody(A0499)

Overview

Product nameMYCN Polyclonal Antibody
Catalog No.A0499
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 168-267 of human MYCN (NP_005369.2).
SequenceAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDED
Gene ID4613
Swiss protP04198
SynonymsMODED; N-myc; NMYC; ODED; bHLHe37; MYCN
Calculated MW49kDa
Observed MW49kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesSH-SY5Y, Mouse heart, Rat brain
Cellular locationNucleus
Customer validation

WB(Human)

ABclonal:Western blot - MYCN Polyclonal Antibody (A0499) }

Western blot - MYCN Polyclonal Antibody (A0499)

Western blot analysis of extracts of various cell lines, using MYCN antibody (A0499) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - MYCN Polyclonal Antibody (A0499) }

Immunohistochemistry - MYCN Polyclonal Antibody (A0499)

Immunohistochemistry of paraffin-embedded mouse brain using MYCN antibody (A0499) (40x lens).
ABclonal:Immunohistochemistry - MYCN Polyclonal Antibody (A0499) }

Immunohistochemistry - MYCN Polyclonal Antibody (A0499)

Immunohistochemistry of paraffin-embedded rat brain using MYCN antibody (A0499) (40x lens).

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