Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

MSL2 Rabbit pAb (A13103)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - MSL2 Rabbit pAb (A13103)

Western blot analysis of various lysates using MSL2 Rabbit pAb (A13103) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Overview

Product name MSL2 Rabbit pAb
Catalog No. A13103
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Predicted to enable ubiquitin protein ligase activity. Involved in histone H4-K16 acetylation. Part of MSL complex.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 328-577 of human MSL2 (NP_060603.2).
Sequence CTAATPKIAKLNRKRSRSESDSEKVQPLPISTIIRGPTLGASAPVTVKRESKISLQPIATVPNGGTTPKISKTVLLSTKSMKKSHEHGSKKSHSKTKPGILKKDKAVKEKIPSHHFMPGSPTKTVYKKPQEKKGCKCGRATQNPSVLTCRGQRCPCYSNRKACLDCICRGCQNSYMANGEKKLEAFAVPEKALEQTRLTLGINVTSIAVRNASTSTSVINVTGSPVTTFLAASTHDDKSLDEAIDMRFDC
Gene ID 55167
Swiss prot Q9HCI7
Synonyms MSL-2; MSL2L1; RNF184; MSL2
Calculated MW 63kDa
Observed MW 62kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HepG2, Mouse brain, Rat liver
Cellular location nucleoplasm, nucleus

MSL2 Rabbit pAb images

ABclonal:Western blot - MSL2 Rabbit pAb (A13103)}

Western blot - MSL2 Rabbit pAb (A13103)

Western blot analysis of various lysates using MSL2 Rabbit pAb (A13103) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on MSL2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MSL2. (Distance between topics and target gene indicate popularity.) MSL2

* Data provided by citexs.com, for reference only.

Publishing research using A13103? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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