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MSH6 Polyclonal Antibody (A3177)

Datasheet

ABclonal:Western blot - MSH6 antibody (A3177)
ABclonal:Immunofluorescence - MSH6 antibody (A3177)
ABclonal:Immunofluorescence - MSH6 antibody (A3177)

Overview

Product nameMSH6 Polyclonal Antibody
Catalog No.A3177
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described.
ImmunogenRecombinant protein of human MSH6
SequenceEmail for sequence
Gene ID2956
Swiss protP52701
SynonymsGTBP; GTMBP; HNPCC5; HSAP; p160
Calculated MW119kDa/120kDa/137kDa/152kDa
Observed MW200kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:1000
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, A-549, MCF-7, Mouse thymus
Cellular locationChromosome, Nucleus
ABclonal:Western blot - MSH6 antibody (A3177)}

Western blot - MSH6 antibody (A3177)

Western blot analysis of extracts of various cell lines, using MSH6 antibody (A3177) at 1:300 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - MSH6 antibody (A3177)}

Immunofluorescence - MSH6 antibody (A3177)

Immunofluorescence analysis of A549 cells using MSH6 antibody (A3177). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MSH6 antibody (A3177)}

Immunofluorescence - MSH6 antibody (A3177)

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using MSH6 antibody (A3177). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

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