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MSH2 Rabbit pAb (A1121)

Datasheet

ABclonal:Western blot - MSH2 Rabbit pAb (A1121)

Western blot analysis of extracts of A-549 cells, using MSH2 Rabbit pAb (A1121) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunoprecipitation - MSH2 Rabbit pAb (A1121)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MSH2 antibody (A1121). Western blot was performed from the immunoprecipitate using MSH2 antibody (A1121) at a dilition of 1:3000.

Overview

Product nameMSH2 Rabbit pAb
Catalog No.A1121
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MSH2 (NP_000242.1).
SequenceMAVQPKETLQLESAAEVGFVRFFQGMPEKPTTTVRLFDRGDFYTAHGEDALLAAREVFKTQGVIKYMGPAGAKNLQSVVLSKMNFESFVKDLLLVRQYRVEVYKNRAGNKASKENDWYLAYKASPGNLSQFEDILFGNNDMSASIGVVGVKMSAVDGQRQVGVGYVDSIQRKLGLCEFPDNDQFSNLEALLIQIGPKECVLPGGETAGDMGKLRQIIQRGGILITERKKADFSTKDIYQDLNRLLKGKKGEQMNSAVLPEMENQVAVSSLSAVIKFLELLSDDSNFGQFELTTFDFSQYM
Gene ID4436
Swiss protP43246
SynonymsMSH2; COCA1; FCC1; HNPCC; HNPCC1; LCFS2
Calculated MW97kDa/104kDa
Observed MW100KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesA-549
Cellular locationNucleus
ABclonal:Western blot - MSH2 Rabbit pAb (A1121) }

Western blot - MSH2 Rabbit pAb (A1121)

Western blot analysis of extracts of A-549 cells, using MSH2 Rabbit pAb (A1121) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunoprecipitation - MSH2 Rabbit pAb (A1121) }

Immunoprecipitation - MSH2 Rabbit pAb (A1121)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MSH2 antibody (A1121). Western blot was performed from the immunoprecipitate using MSH2 antibody (A1121) at a dilition of 1:3000.

* For research use only. Not for therapeutic or diagnostic purposes.

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