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MSH2 Polyclonal Antibody (A1121)

Datasheet

ABclonal:Western blot - MSH2 Polyclonal Antibody (A1121)
ABclonal:Immunoprecipitation - MSH2 Polyclonal Antibody (A1121)

Overview

Product nameMSH2 Polyclonal Antibody
Catalog No.A1121
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MSH2 (NP_000242.1).
SequenceMAVQPKETLQLESAAEVGFVRFFQGMPEKPTTTVRLFDRGDFYTAHGEDALLAAREVFKTQGVIKYMGPAGAKNLQSVVLSKMNFESFVKDLLLVRQYRVEVYKNRAGNKASKENDWYLAYKASPGNLSQFEDILFGNNDMSASIGVVGVKMSAVDGQRQVGVGYVDSIQRKLGLCEFPDNDQFSNLEALLIQIGPKECVLPGGETAGDMGKLRQIIQRGGILITERKKADFSTKDIYQDLNRLLKGKKGEQMNSAVLPEMENQVAVSSLSAVIKFLELLSDDSNFGQFELTTFDFSQYM
Gene ID4436
Swiss protP43246
SynonymsCOCA1; FCC1; HNPCC; HNPCC1; LCFS2
Calculated MW97kDa/104kDa
Observed MW105kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesNIH/3T3, A431, A549, Mouse testis, Mouse thymus, Mouse brain, Rat testis
Cellular locationNucleus
ABclonal:Western blot - MSH2 Polyclonal Antibody (A1121) }

Western blot - MSH2 Polyclonal Antibody (A1121)

Western blot analysis of extracts of various cell lines, using MSH2 antibody (A1121) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunoprecipitation - MSH2 Polyclonal Antibody (A1121) }

Immunoprecipitation - MSH2 Polyclonal Antibody (A1121)

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug MSH2 antibody (A1121). Western blot was performed from the immunoprecipitate using MSH2 antibody (A1121) at a dilition of 1:1000.

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