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MRP4 / ABCC4 Polyclonal Antibody (A2198)

Datasheet

ABclonal:Western blot - MRP4 / ABCC4 Polyclonal Antibody (A2198)
ABclonal:Immunofluorescence - MRP4 / ABCC4 Polyclonal Antibody (A2198)

Overview

Product nameMRP4 / ABCC4 Polyclonal Antibody
Catalog No.A2198
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This family member plays a role in cellular detoxification as a pump for its substrate, organic anions. It may also function in prostaglandin-mediated cAMP signaling in ciliogenesis. Alternative splicing of this gene results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 495-705 of human MRP4 / ABCC4 (NP_001098985.1).
SequenceFGKKYEKERYEKVIKACALKKDLQLLEDGDLTVIGDRGTTLSGGQKARVNLARAVYQDADIYLLDDPLSAVDAEVSRHLFELCICQILHEKITILVTHQLQYLKAASQILILKDGKMVQKGTYTEFLKSGIDFGSLLKKDNEESEQPPVPGTPTLRNRTFSESSVWSQQSSRPSLKDGALESQDTENVPVTLSEENRSEGKVGFQAYKNYF
Gene ID10257
Swiss protO15439
SynonymsABCC4; MOAT-B; MOATB; MRP4; multidrug resistance-associated protein 4
Calculated MW88kDa/96kDa/144kDa/149kDa
Observed MW170kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesDU145, 293T
Cellular locationMembrane, Multi-pass membrane protein
Customer validation

WB(Mouse)

ABclonal:Western blot - MRP4 / ABCC4 Polyclonal Antibody (A2198) }

Western blot - MRP4 / ABCC4 Polyclonal Antibody (A2198)

Western blot analysis of extracts of various cell lines, using MRP4 / ABCC4 antibody (A2198) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - MRP4 / ABCC4 Polyclonal Antibody (A2198) }

Immunofluorescence - MRP4 / ABCC4 Polyclonal Antibody (A2198)

Immunofluorescence analysis of HeLa cells using MRP4 / ABCC4 antibody (A2198) at dilution of 1:100. Blue: DAPI for nuclear staining.

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