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MRP2/ABCC2 Rabbit pAb (A8405)

Publications (3) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - MRP2/ABCC2 Rabbit pAb (A8405)

Western blot analysis of extracts of various cell lines, using MRP2/ABCC2 antibody (A8405) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - MRP2/ABCC2 Rabbit pAb (A8405)

Western blot analysis of extracts of various cell lines, using MRP2/ABCC2 antibody (A8405) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameMRP2/ABCC2 Rabbit pAb
Catalog No.A8405
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1470-1545 of human MRP2/ABCC2 (NP_000383.2).
SequenceETDNLIQTTIQNEFAHCTVITIAHRLHTIMDSDKVMVLDNGKIIECGSPEELLQIPGPFYFMAKEAGIENVNSTKF
Gene ID1244
Swiss protQ92887
SynonymsABCC2; ABC30; CMOAT; DJS; MRP2; cMRP
Calculated MW174kDa
Observed MW200KDa-270KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHepG2, A549, Mouse lung, Rat lung, Rat kidney
Cellular locationApical cell membrane, Multi-pass membrane protein
Customer validation

WB(Homo sapiens,Oryza sativa, Plutella xylostella)

IF(Homo sapiens)

ABclonal:Western blot - MRP2/ABCC2 Rabbit pAb (A8405) }

Western blot - MRP2/ABCC2 Rabbit pAb (A8405)

Western blot analysis of extracts of various cell lines, using MRP2/ABCC2 antibody (A8405) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - MRP2/ABCC2 Rabbit pAb (A8405) }

Western blot - MRP2/ABCC2 Rabbit pAb (A8405)

Western blot analysis of extracts of various cell lines, using MRP2/ABCC2 antibody (A8405) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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