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MRP1/ABCC1 Rabbit pAb (A3027)

Publication (1) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - MRP1/ABCC1 Rabbit pAb (A3027)

Western blot analysis of extracts of various cell lines, using MRP1/ABCC1 antibody (A3027) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - MRP1/ABCC1 Rabbit pAb (A3027)

Western blot analysis of extracts of A-549 cells, using MRP1/ABCC1 antibody (A3027) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunofluorescence - MRP1/ABCC1 Rabbit pAb (A3027)

Immunofluorescence analysis of HepG2 cells using MRP1/ABCC1 Rabbit pAb (A3027) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameMRP1/ABCC1 Rabbit pAb
Catalog No.A3027
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 210-304 of human MRP1/ABCC1 (NP_004987.2).
SequenceESSASFLSRITFWWITGLIVRGYRQPLEGSDLWSLNKEDTSEQVVPVLVKNWKKECAKTRKQPVKVVYSSKDPAQPKESSKVDANEEVEALIVKS
Gene ID4363
Swiss protP33527
SynonymsABCC1; ABC29; ABCC; GS-X; MRP; MRP1
Calculated MW151-172kDa
Observed MW170-220KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549, Mouse brain, Rat brain
Cellular locationCell membrane, Multi-pass membrane protein
Customer validation

WB(Streptomyces sp. FJS31-2)

ABclonal:Western blot - MRP1/ABCC1 Rabbit pAb (A3027) }

Western blot - MRP1/ABCC1 Rabbit pAb (A3027)

Western blot analysis of extracts of various cell lines, using MRP1/ABCC1 antibody (A3027) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - MRP1/ABCC1 Rabbit pAb (A3027) }

Western blot - MRP1/ABCC1 Rabbit pAb (A3027)

Western blot analysis of extracts of A-549 cells, using MRP1/ABCC1 antibody (A3027) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunofluorescence - MRP1/ABCC1 Rabbit pAb (A3027) }

Immunofluorescence - MRP1/ABCC1 Rabbit pAb (A3027)

Immunofluorescence analysis of HepG2 cells using MRP1/ABCC1 Rabbit pAb (A3027) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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