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MMP9 Rabbit pAb (A0289)

Reviews (4)Publications (27) Datasheet

ABclonal:MMP9 Rabbit pAb
ABclonal:Western blot - MMP9 Rabbit pAb (A0289)

Western blot analysis of extracts of various cell lines, using MMP9 Rabbit pAb (A0289) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - MMP9 Rabbit pAb (A0289)

Immunohistochemistry of paraffin-embedded rat spleen using MMP9 antibody (A0289) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - MMP9 Rabbit pAb (A0289)

Immunohistochemistry of paraffin-embedded mouse testis using MMP9 antibody (A0289) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - MMP9 Rabbit pAb (A0289)

Immunohistochemistry of paraffin-embedded mouse spleen using MMP9 antibody (A0289) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - MMP9 Rabbit pAb (A0289)

Immunofluorescence analysis of L929 cells using MMP9 antibody (A0289) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for MMP9 Rabbit pAb(A0289)
Review
ABclonal: review for MMP9 Rabbit pAb(A0289)
Review
ABclonal: review for MMP9 Rabbit pAb(A0289)
Review
ABclonal: review for MMP9 Rabbit pAb(A0289)

Overview

Product nameMMP9 Rabbit pAb
Catalog No.A0289
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 538-707 of human MMP9 (NP_004985.2).
SequenceKYWRFSEGRGSRPQGPFLIADKWPALPRKLDSVFEERLSKKLFFFSGRQVWVYTGASVLGPRRLDKLGLGADVAQVTGALRSGRGKMLLFSGRRLWRFDVKAQMVDPRSASEVDRMFPGVPLDTHDVFQYREKAYFCQDRFYWRVSSRSELNQVDQVGYVTYDILQCPED
Gene ID4318
Swiss protP14780
SynonymsMMP9; CLG4B; GELB; MANDP2; MMP-9
Calculated MW78kDa
Observed MW84KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, NIH/3T3, Jurkat, K-562, Raji, Mouse brain, Rat kidney
Cellular locationSecreted, extracellular matrix, extracellular space
Customer validation

(Eoma cell, Human glioma tissue, MDA-MB-231)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus)

IHC(Mus musculus)

IHC(Gallus gallus)

ABclonal:Western blot - MMP9 Rabbit pAb (A0289) }

Western blot - MMP9 Rabbit pAb (A0289)

Western blot analysis of extracts of various cell lines, using MMP9 Rabbit pAb (A0289) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - MMP9 Rabbit pAb (A0289) }

Immunohistochemistry - MMP9 Rabbit pAb (A0289)

Immunohistochemistry of paraffin-embedded rat spleen using MMP9 antibody (A0289) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MMP9 Rabbit pAb (A0289) }

Immunohistochemistry - MMP9 Rabbit pAb (A0289)

Immunohistochemistry of paraffin-embedded mouse testis using MMP9 antibody (A0289) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MMP9 Rabbit pAb (A0289) }

Immunohistochemistry - MMP9 Rabbit pAb (A0289)

Immunohistochemistry of paraffin-embedded mouse spleen using MMP9 antibody (A0289) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - MMP9 Rabbit pAb (A0289) }

Immunofluorescence - MMP9 Rabbit pAb (A0289)

Immunofluorescence analysis of L929 cells using MMP9 antibody (A0289) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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