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MMP3 Rabbit pAb (A1202)

Review (1)Publications (4) Datasheet

ABclonal:Western blot - MMP3 Rabbit pAb (A1202)

Western blot analysis of extracts of various cell lines, using MMP3 antibody (A1202) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - MMP3 Rabbit pAb (A1202)

Western blot analysis of extracts of Rat liver, using MMP3 antibody (A1202) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - MMP3 Rabbit pAb (A1202)

Immunohistochemistry of paraffin-embedded human breast cancer using MMP3 Rabbit pAb (A1202) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - MMP3 Rabbit pAb (A1202)

Immunofluorescence analysis of C6 cells using MMP3 Polyclonal Antibody (A1202) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MMP3 Rabbit pAb (A1202)

Immunofluorescence analysis of U-2 OS cells using MMP3 Polyclonal Antibody (A1202) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for MMP3 Rabbit pAb(A1202)

Overview

Product nameMMP3 Rabbit pAb
Catalog No.A1202
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 248-477 of human MMP3 (NP_002413.1).
SequenceRFRLSQDDINGIQSLYGPPPDSPETPLVPTEPVPPEPGTPANCDPALSFDAVSTLRGEILIFKDRHFWRKSLRKLEPELHLISSFWPSLPSGVDAAYEVTSKDLVFIFKGNQFWAIRGNEVRAGYPRGIHTLGFPPTVRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNC
Gene ID4314
Swiss protP08254
SynonymsMMP3; CHDS6; MMP-3; SL-1; STMY; STMY1; STR1
Calculated MW53kDa
Observed MW60KDa/47KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRaji, Mouse heart, Rat liver
Cellular locationSecreted, extracellular matrix, extracellular space
Customer validation

(MB-231)

WB(Homo sapiens, Rattus norvegicus)

ABclonal:Western blot - MMP3 Rabbit pAb (A1202) }

Western blot - MMP3 Rabbit pAb (A1202)

Western blot analysis of extracts of various cell lines, using MMP3 antibody (A1202) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - MMP3 Rabbit pAb (A1202) }

Western blot - MMP3 Rabbit pAb (A1202)

Western blot analysis of extracts of Rat liver, using MMP3 antibody (A1202) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - MMP3 Rabbit pAb (A1202) }

Immunohistochemistry - MMP3 Rabbit pAb (A1202)

Immunohistochemistry of paraffin-embedded human breast cancer using MMP3 Rabbit pAb (A1202) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - MMP3 Rabbit pAb (A1202) }

Immunofluorescence - MMP3 Rabbit pAb (A1202)

Immunofluorescence analysis of C6 cells using MMP3 Polyclonal Antibody (A1202) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MMP3 Rabbit pAb (A1202) }

Immunofluorescence - MMP3 Rabbit pAb (A1202)

Immunofluorescence analysis of U-2 OS cells using MMP3 Polyclonal Antibody (A1202) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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