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MMP2 Rabbit pAb (A6247)

Publications (14) Datasheet

ABclonal:Western blot - MMP2 Polyclonal Antibody (A6247)
ABclonal:Immunohistochemistry - MMP2 Polyclonal Antibody (A6247)

Overview

Product nameMMP2 Rabbit pAb
Catalog No.A6247
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 40-280 of human MMP2 (NP_004521.1).
SequenceDVAPKTDKELAVQYLNTFYGCPKESCNLFVLKDTLKKMQKFFGLPQTGDLDQNTIETMRKPRCGNPDVANYNFFPRKPKWDKNQITYRIIGYTPDLDPETVDDAFARAFQVWSDVTPLRFSRIHDGEADIMINFGRWEHGDGYPFDGKDGLLAHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVKYGNADGEYCKFPFLFNGKEYNSCTDTGRSDGFLWCSTTYNFEKDGKYGFCPHEALF
Gene ID4313
Swiss protP08253
SynonymsCLG4; CLG4A; MMP-2; MMP-II; MONA; TBE-1; MMP2
Calculated MW65kDa/68kDa/73kDa
Observed MW73kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, A375, Mouse liver
Cellular locationCytoplasm, Membrane, Mitochondrion, Nucleus, Secreted, extracellular matrix, extracellular space
Customer validation

WB(Rattus norvegicus, Homo sapiens, Mus musculus)

IHC(Homo sapiens)

WB(Homo sapiens)

IHC(Rattus norvegicus, Homo sapiens)

ABclonal:Western blot - MMP2 Polyclonal Antibody (A6247) }

Western blot - MMP2 Polyclonal Antibody (A6247)

Western blot analysis of extracts of various cell lines, using MMP2 antibody (A6247) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - MMP2 Polyclonal Antibody (A6247) }

Immunohistochemistry - MMP2 Polyclonal Antibody (A6247)

Immunohistochemistry of paraffin-embedded human placenta using MMP2 antibody (A6247) at dilution of 1:100 (40x lens).

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