Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

MLH3 Rabbit pAb (A7796)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - MLH3 Rabbit pAb (A7796)

Western blot analysis of HCT116, using MLH3 Rabbit pAb (A7796) at 1:3500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Overview

Product name MLH3 Rabbit pAb
Catalog No. A7796
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1190-1389 of human MLH3 (NP_055196.2).
Sequence QVLQQVDNKFIACLMSTKTEENGEADSYEKQQAQGSGRKKLLSSTLIPPLEITVTEEQRRLLWCYHKNLEDLGLEFVFPDTSDSLVLVGKVPLCFVEREANELRRGRSTVTKSIVEEFIREQLELLQTTGGIQGTLPLTVQKVLASQACHGAIKFNDGLSLQESCRLIEALSSCQLPFQCAHGRPSMLPLADIDHLEQEK
Gene ID 27030
Swiss prot Q9UHC1
Synonyms HNPCC7; MLH3
Calculated MW 164kDa
Observed MW 164kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HCT116
Cellular location Nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

MLH3 Rabbit pAb images

ABclonal:Western blot - MLH3 Rabbit pAb (A7796)}

Western blot - MLH3 Rabbit pAb (A7796)

Western blot analysis of HCT116, using MLH3 Rabbit pAb (A7796) at 1:3500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Related Targets

Based on existing publications, the following genes are closely related to research on MLH3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MLH3. (Distance between topics and target gene indicate popularity.) MLH3

* Data provided by citexs.com, for reference only.

Publishing research using A7796? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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