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MFN1 Rabbit pAb (A9880)

Reviews (2)Publications (11) Datasheet

ABclonal:Western blot - MFN1 Rabbit pAb (A9880)

Western blot analysis of extracts of various cell lines, using MFN1 antibody (A9880) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Review
ABclonal: review for MFN1 Rabbit pAb(A9880)
Review
ABclonal: review for MFN1 Rabbit pAb(A9880)

Overview

Product nameMFN1 Rabbit pAb
Catalog No.A9880
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 622-741 of human MFN1 (NP_284941.2).
SequenceYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEEEIARLPKEIDQLEKIQNNSKLLRNKAVQLENELENFTKQFLPSSNEES
Gene ID55669
Swiss protQ8IWA4
SynonymsMFN1; hfzo1; hfzo2
Calculated MW41kDa/71kDa/84kDa
Observed MW84KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, PC-3, Rat heart
Cellular locationCytoplasm, Mitochondrion outer membrane, Multi-pass membrane protein
Customer validation

(Human bone marrow cancer cell)

WB(Rattus norvegicus, Homo sapiens, Gallus gallus, Mus musculus)

ABclonal:Western blot - MFN1 Rabbit pAb (A9880) }

Western blot - MFN1 Rabbit pAb (A9880)

Western blot analysis of extracts of various cell lines, using MFN1 antibody (A9880) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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