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MFGE8 Rabbit pAb (A12322)

Publications (2) Datasheet

ABclonal:Western blot - MFGE8 Rabbit pAb (A12322)

Western blot analysis of extracts of various cell lines, using MFGE8 antibody (A12322) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - MFGE8 Rabbit pAb (A12322)

Immunohistochemistry of paraffin-embedded Rat lung using MFGE8 antibody (A12322) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - MFGE8 Rabbit pAb (A12322)

Immunohistochemistry of paraffin-embedded Human lung using MFGE8 antibody (A12322) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - MFGE8 Rabbit pAb (A12322)

Immunohistochemistry of paraffin-embedded Human mammary cancer using MFGE8 antibody (A12322) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - MFGE8 Rabbit pAb (A12322)

Immunohistochemistry of paraffin-embedded Mouse lung using MFGE8 antibody (A12322) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - MFGE8 Rabbit pAb (A12322)

Immunofluorescence analysis of C6 cells using MFGE8 antibody (A12322) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MFGE8 Rabbit pAb (A12322)

Immunofluorescence analysis of L929 cells using MFGE8 antibody (A12322) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MFGE8 Rabbit pAb (A12322)

Immunofluorescence analysis of U-2 OS cells using MFGE8 antibody (A12322) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameMFGE8 Rabbit pAb
Catalog No.A12322
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human MFGE8 (NP_001108086.1).
SequenceMPRPRLLAALCGALLCAPSLLVALDICSKNPCHNGGLCEEISQEVRGDVFPSYTCTCLKGYAGNHCETKCVEPLGLENGNIANSQIAASSVRVTFLGLQHWVPELARLNRAGMVNAWTPSSNDDNPWIQVNLLRRMWVTGVVTQGASRLASHEYLKAFKVAYSLNGHEFDFIHDVNKKHKEFVGNWNKNAVHVNLFETPV
Gene ID4240
Swiss protQ08431
SynonymsMFGE8; BA46; EDIL1; HMFG; HsT19888; MFG-E8; MFGM; OAcGD3S; SED1; SPAG10; hP47
Calculated MW35kDa/37kDa/43kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse heart, Rat heart
Cellular locationMembrane, Peripheral membrane protein, Secreted
Customer validation

WB(Mus musculus)

IF(Mus musculus)

IHC(Homo sapiens,Mus musculus)

IF(Homo sapiens,Mus musculus)

ABclonal:Western blot - MFGE8 Rabbit pAb (A12322) }

Western blot - MFGE8 Rabbit pAb (A12322)

Western blot analysis of extracts of various cell lines, using MFGE8 antibody (A12322) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - MFGE8 Rabbit pAb (A12322) }

Immunohistochemistry - MFGE8 Rabbit pAb (A12322)

Immunohistochemistry of paraffin-embedded Rat lung using MFGE8 antibody (A12322) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MFGE8 Rabbit pAb (A12322) }

Immunohistochemistry - MFGE8 Rabbit pAb (A12322)

Immunohistochemistry of paraffin-embedded Human lung using MFGE8 antibody (A12322) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MFGE8 Rabbit pAb (A12322) }

Immunohistochemistry - MFGE8 Rabbit pAb (A12322)

Immunohistochemistry of paraffin-embedded Human mammary cancer using MFGE8 antibody (A12322) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MFGE8 Rabbit pAb (A12322) }

Immunohistochemistry - MFGE8 Rabbit pAb (A12322)

Immunohistochemistry of paraffin-embedded Mouse lung using MFGE8 antibody (A12322) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - MFGE8 Rabbit pAb (A12322) }

Immunofluorescence - MFGE8 Rabbit pAb (A12322)

Immunofluorescence analysis of C6 cells using MFGE8 antibody (A12322) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MFGE8 Rabbit pAb (A12322) }

Immunofluorescence - MFGE8 Rabbit pAb (A12322)

Immunofluorescence analysis of L929 cells using MFGE8 antibody (A12322) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MFGE8 Rabbit pAb (A12322) }

Immunofluorescence - MFGE8 Rabbit pAb (A12322)

Immunofluorescence analysis of U-2 OS cells using MFGE8 antibody (A12322) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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