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MDM2 Rabbit pAb (A0345)

Publications (3) Datasheet

ABclonal:Western blot - MDM2 Rabbit pAb (A0345)

Western blot analysis of extracts of various cell lines, using MDM2 antibody (A0345) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - MDM2 Rabbit pAb (A0345)

Immunohistochemistry of paraffin-embedded rat kidney using MDM2 Antibody (A0345) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - MDM2 Rabbit pAb (A0345)

Immunohistochemistry of paraffin-embedded human colon carcinoma using MDM2 Antibody (A0345) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - MDM2 Rabbit pAb (A0345)

Immunohistochemistry of paraffin-embedded mouse brain using MDM2 Antibody (A0345) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - MDM2 Rabbit pAb (A0345)

Immunofluorescence analysis of A549 cells using MDM2 antibody (A0345). Blue: DAPI for nuclear staining.

Overview

Product nameMDM2 Rabbit pAb
Catalog No.A0345
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 130-230 of human MDM2 (NP_002383.2).
SequenceENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDL
Gene ID4193
Swiss protQ00987
SynonymsMDM2; ACTFS; HDMX; hdm2
Calculated MW11-14kDa/24-55kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSKOV3, HT-29, Jurkat
Cellular locationCytoplasm, Nucleus, nucleolus, nucleoplasm
Customer validation

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - MDM2 Rabbit pAb (A0345) }

Western blot - MDM2 Rabbit pAb (A0345)

Western blot analysis of extracts of various cell lines, using MDM2 antibody (A0345) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - MDM2 Rabbit pAb (A0345) }

Immunohistochemistry - MDM2 Rabbit pAb (A0345)

Immunohistochemistry of paraffin-embedded rat kidney using MDM2 Antibody (A0345) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - MDM2 Rabbit pAb (A0345) }

Immunohistochemistry - MDM2 Rabbit pAb (A0345)

Immunohistochemistry of paraffin-embedded human colon carcinoma using MDM2 Antibody (A0345) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - MDM2 Rabbit pAb (A0345) }

Immunohistochemistry - MDM2 Rabbit pAb (A0345)

Immunohistochemistry of paraffin-embedded mouse brain using MDM2 Antibody (A0345) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - MDM2 Rabbit pAb (A0345) }

Immunofluorescence - MDM2 Rabbit pAb (A0345)

Immunofluorescence analysis of A549 cells using MDM2 antibody (A0345). Blue: DAPI for nuclear staining.

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