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MCP-1 Rabbit pAb (A7277)

Review (1)Publications (8) Datasheet

ABclonal:Western blot - MCP-1 Rabbit pAb (A7277)

Western blot analysis of extracts of various cell lines, using MCP-1 antibody (A7277) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - MCP-1 Rabbit pAb (A7277)

Immunohistochemistry of paraffin-embedded human colon using MCP-1 antibody (A7277) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - MCP-1 Rabbit pAb (A7277)

Immunohistochemistry of paraffin-embedded human stomach using MCP-1 antibody (A7277) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - MCP-1 Rabbit pAb (A7277)

Immunohistochemistry of paraffin-embedded mouse heart using MCP-1 antibody (A7277) at dilution of 1:100 (40x lens).

Review
ABclonal: review for MCP-1 Rabbit pAb(A7277)

Overview

Product nameMCP-1 Rabbit pAb
Catalog No.A7277
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-99 of human MCP-1 (NP_002973.1).
SequenceQPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT
Gene ID6347
Swiss protP13500
SynonymsCCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF
Calculated MW11kDa
Observed MW13KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMouse lung, Mouse brain, Rat lung, Rat kidney
Cellular locationSecreted
Customer validation

(rat brain cells)

IHC(Mus musculus)

WB(Mus musculus, Rattus norvegicus, Homo sapiens)

IHC(Mus musculus, Rattus norvegicus)

ABclonal:Western blot - MCP-1 Rabbit pAb (A7277) }

Western blot - MCP-1 Rabbit pAb (A7277)

Western blot analysis of extracts of various cell lines, using MCP-1 antibody (A7277) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - MCP-1 Rabbit pAb (A7277) }

Immunohistochemistry - MCP-1 Rabbit pAb (A7277)

Immunohistochemistry of paraffin-embedded human colon using MCP-1 antibody (A7277) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MCP-1 Rabbit pAb (A7277) }

Immunohistochemistry - MCP-1 Rabbit pAb (A7277)

Immunohistochemistry of paraffin-embedded human stomach using MCP-1 antibody (A7277) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MCP-1 Rabbit pAb (A7277) }

Immunohistochemistry - MCP-1 Rabbit pAb (A7277)

Immunohistochemistry of paraffin-embedded mouse heart using MCP-1 antibody (A7277) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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