Review (1)Publications (8) Datasheet
Western blot analysis of extracts of various cell lines, using MCP-1 antibody (A7277) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry of paraffin-embedded human colon using MCP-1 antibody (A7277) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MCP-1 antibody (A7277) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using MCP-1 antibody (A7277) at dilution of 1:100 (40x lens).
| Product name | MCP-1 Rabbit pAb |
|---|---|
| Catalog No. | A7277 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 24-99 of human MCP-1 (NP_002973.1). |
|---|---|
| Sequence | QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT |
| Gene ID | 6347 |
| Swiss prot | P13500 |
| Synonyms | CCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF |
| Calculated MW | 11kDa |
| Observed MW | 13KDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry |
| Positive samples | Mouse lung, Mouse brain, Rat lung, Rat kidney |
| Cellular location | Secreted |
| Customer validation | (rat brain cells) IHC(Mus musculus) WB(Mus musculus, Rattus norvegicus, Homo sapiens) IHC(Mus musculus, Rattus norvegicus) |
* For research use only. Not for therapeutic or diagnostic purposes.
Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com
Submit your question about A7277 below and we will get back to you within one business day.
Alternatively, call us at 888.754.5670.
Publishing research using A7277? Please let us know so that we can cite the reference in this datasheet.
