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CCL2/MCP-1 Rabbit pAb (A7277)

Review (1)Publications (17) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - CCL2/MCP-1 Rabbit pAb (A7277)

Western blot analysis of extracts of Rat kidney, using CCL2/MCP-1 antibody (A7277) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Review
ABclonal: review for CCL2/MCP-1 Rabbit pAb(A7277)

Overview

Product nameCCL2/MCP-1 Rabbit pAb
Catalog No.A7277
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-99 of human CCL2/MCP-1 (NP_002973.1).
SequenceQPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT
Gene ID6347
Swiss protP13500
SynonymsCCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF
Calculated MW11kDa
Observed MW13KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesRat kidney
Cellular locationSecreted
Customer validation

(rat brain cells)

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

IHC(Rattus norvegicus, Mus musculus)

IHC(Mus musculus, Homo sapiens)

ABclonal:Western blot - CCL2/MCP-1 Rabbit pAb (A7277)}

Western blot - CCL2/MCP-1 Rabbit pAb (A7277)

Western blot analysis of extracts of Rat kidney, using CCL2/MCP-1 antibody (A7277) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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