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CCL2/MCP-1 Rabbit pAb (A7277)

Tested applications:WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat

Western blot analysis of extracts of Rat kidney, using CCL2/MCP-1 antibody (A7277) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Review

Overview

Product name	CCL2/MCP-1 Rabbit pAb
Catalog No.	A7277
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 24-99 of human CCL2/MCP-1 (NP_002973.1).
Sequence	QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT
Gene ID	6347
Swiss prot	P13500
Synonyms	CCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF
Calculated MW	11kDa
Observed MW	13KDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting
Positive samples	Rat kidney
Cellular location	Secreted
Customer validation	(rat brain cells) WB(Mus musculus, Homo sapiens, Rattus norvegicus) IHC(Rattus norvegicus, Mus musculus) IHC(Mus musculus, Homo sapiens)

Research Area

CCL2/MCP-1 Rabbit pAb images

Western blot - CCL2/MCP-1 Rabbit pAb (A7277)

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References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

Vascular Pharmacology

Application:

IF:

5.773

Species:

Mus musculus

PMID:

28069546

Title:

G004, a synthetic sulfonylurea compound, exerts anti-atherosclerosis effects by targeting SIRT1 in ApoE(-/-) mice

References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

tumor biology

Application:

IF:

3.355

Species:

Homo sapiens

PMID:

28347237

Title:

Specific tumor-derived CCL2 mediated by pyruvate kinase M2 in colorectal cancer cells contributes to macrophage recruitment in tumor microenvironment

References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

Bioscience reports

Application:

WB, IHC

IF:

3.84

Species:

Rattus norvegicus

PMID:

30760630

Title:

Increase of glandular epithelial cell clusters by an external volume expansion device promotes adipose tissue regeneration by recruiting macrophages.

References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

Inflammopharmacology

Application:

WB, IHC

IF:

4.473

Species:

Mus musculus

PMID:

31938969

Title:

Human umbilical cord mesenchymal stem cells alleviate inflammatory bowel disease by inhibiting ERK phosphorylation in neutrophils

References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

Frontiers in Pharmacology

Application:

IHC

IF:

5.81

Species:

Mus musculus

PMID:

32116720

Title:

Alleviation of Synovial Inflammation of Juanbi-Tang on Collagen-Induced Arthritis and TNF-Tg Mice Model

References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

Life Sciences

Application:

IF:

5.037

Species:

Mus musculus

PMID:

32417373

Title:

CREBH knockout accelerates hepatic fibrosis in mouse models of diet-induced nonalcoholic fatty liver disease

References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

Cancers

Application:

IHC

IF:

6.639

Species:

Mus musculus

PMID:

33260558

Title:

Lung Cancer Cell-Derived Secretome Mediates Paraneoplastic Inflammation and Fibrosis in Kidney in Mice

References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

Life Sci

Application:

IF:

5.037

Species:

Rattus norvegicus

PMID:

33577853

Title:

Neuroprotective effect of paeonol in streptozotocin-induced diabetes in rats

References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

Physiol Rep

Application:

IF:

Species:

Mus musculus

PMID:

34435466

Title:

Differential inflammatory responses of the native left and right ventricle associated with donor heart preservation

References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

Cell Death Discov

Application:

IHC

IF:

5.241

Species:

Mus musculus

PMID:

34455423

Title:

ARRB1 suppresses the activation of hepatic macrophages via modulating endoplasmic reticulum stress in lipopolysaccharide-induced acute liver injury

References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

Free Radic Biol Med

Application:

IHC

IF:

7.376

Species:

Mus musculus

PMID:

34474107

Title:

Elevated branched-chain α-keto acids exacerbate macrophage oxidative stress and chronic inflammatory damage in type 2 diabetes mellitus

References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

Acta Histochem

Application:

IF:

2.479

Species:

Rattus norvegicus

PMID:

34517258

Title:

Protective action of ultrasound-guided intraparenchymal transplantation of BMSCs in adriamycin nephropathy rats through the RIPK3/MLKL and NLRP3 pathways

References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

Cancer Invest

Application:

IHC

IF:

2.176

Species:

Homo sapiens,Mus musculus

PMID:

34515610

Title:

Surgical Trauma-Induced CCL2 Upregulation Mediates Lung Cancer Progression by Promoting Treg Recruitment in Mice and Patients

References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

J Periodontal Res

Application:

WB, IHC

IF:

4.419

Species:

Rattus norvegicus

PMID:

34773653

Title:

Activation of GATA‐binding protein 4 regulates monocyte chemoattractant protein‐1 and chemotaxis in periodontal ligament cells

References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico

Application:

IF:

3.4

Species:

Homo sapiens

PMID:

33405050

Title:

LINC01255 combined with BMI1 to regulate human mesenchymal stromal senescence and acute myeloid leukemia cell proliferation through repressing transcription of MCP-1

References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

Bioactive materials

Application:

IF:

14.59

Species:

Mus musculus,Homo sapiens

PMID:

35386458

Title:

Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis

References for CCL2/MCP-1 Rabbit pAb (A7277)

Product:

CCL2/MCP-1 Rabbit pAb

Journal:

Experimental animals

Application:

IF:

Species:

Mus musculus

PMID:

35491099

Title:

Suppression of lysosomal-associated protein transmembrane 5 ameliorates cardiac function and inflammatory response by inhibiting the NF-κB pathway after myocardial infarction in mice