Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

MCCC2 Rabbit pAb (A15181)

Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - MCCC2 Rabbit pAb (A15181)

Western blot analysis of extracts of various cell lines, using MCCC2 Rabbit pAb (A15181) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - MCCC2 Rabbit pAb (A15181)

Immunofluorescence analysis of U2OS cells using MCCC2 antibody (A15181) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameMCCC2 Rabbit pAb
Catalog No.A15181
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.

Immunogen information

ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 284-563 of human MCCC2 (NP_071415.1).
SequenceLHLTRKVVRNLNYQKKLDVTIEPSEEPLFPADELYGIVGANLKRSFDVREVIARIVDGSRFTEFKAFYGDTLVTGFARIFGYPVGIVGNNGVLFSESAKKGTHFVQLCCQRNIPLLFLQNITGFMVGREYEAEGIAKDGAKMVAAVACAQVPKITLIIGGSYGAGNYGMCGRAYSPRFLYIWPNARISVMGGEQAANVLATITKDQRAREGKQFSSADEAALKEPIIKKFEEEGNPYYSSARVWDDGIIDPADTRLVLGLSFSAALNAPIEKTDFGIFRM
Gene ID64087
Swiss protQ9HCC0
SynonymsMCCC2; MCCB
Calculated MW57kDa/61kDa
Observed MW61kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    Immunohistochemistry    
Positive samples22Rv1, HeLa, HepG2, U-251MG, Mouse liver, Mouse kidney
Cellular locationMitochondrion matrix

Research Area

MCCC2 Rabbit pAb images

ABclonal:Western blot - MCCC2 Rabbit pAb (A15181)}

Western blot - MCCC2 Rabbit pAb (A15181)

Western blot analysis of extracts of various cell lines, using MCCC2 Rabbit pAb (A15181) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - MCCC2 Rabbit pAb (A15181)}

Immunofluorescence - MCCC2 Rabbit pAb (A15181)

Immunofluorescence analysis of U2OS cells using MCCC2 antibody (A15181) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on MCCC2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MCCC2. (Distance between topics and target gene indicate popularity.) MCCC2

* Data provided by citexs.com, for reference only.

Publishing research using A15181? Please let us know so that we can cite the reference in this datasheet.

Primary Antibodies (1)

Antibody Duos (1)

Secondary Antibodies (26)

* For research use only. Not for therapeutic or diagnostic purposes.

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