Products > Catalog Antibodies > Polyclonal Antibodies

MAPK3 Polyclonal Antibody (A0228)

Publications (8) Datasheet

ABclonal:Western blot - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Overview

Product nameMAPK3 Polyclonal Antibody
Catalog No.A0228
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
ImmunogenA synthetic peptide of human MAPK3
SequenceEmail for sequence
Gene ID5595
Swiss protP27361
SynonymsERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM3
Calculated MW38kDa/40kDa/43kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesDU145, HeLa
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Human, Murine)

ABclonal:Western blot - MAPK3 Polyclonal Antibody (A0228) }

Western blot - MAPK3 Polyclonal Antibody (A0228)

Western blot analysis of extracts of various cell lines, using MAPK3 antibody (A0228) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded rat kidney using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded rat heart using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded human kidney using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded mouse testis using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded mouse heart using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded rat lung using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded rat liver using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded rat skeletal muscle using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded rat heart using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded human liver using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded human kidney using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded human kidney cancer using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded mouse lung using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded mouse liver using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded mouse testis using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded mouse brain using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded mouse kidney using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228) }

Immunohistochemistry - MAPK3 Polyclonal Antibody (A0228)

Immunohistochemistry of paraffin-embedded mouse heart using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).

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