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MAPK3 Rabbit pAb (A0228)

Publications (8) Datasheet

ABclonal:Western blot - MAPK3 Rabbit pAb (A0228)
ABclonal:Western blot - MAPK3 Rabbit pAb (A0228)
ABclonal:Immunohistochemistry - MAPK3 Rabbit pAb (A0228)
ABclonal:Immunohistochemistry - MAPK3 Rabbit pAb (A0228)
ABclonal:Immunohistochemistry - MAPK3 Rabbit pAb (A0228)

Overview

Product nameMAPK3 Rabbit pAb
Catalog No.A0228
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAPK3 (NP_002737.2).
SequenceMAAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENV
Gene ID5595
Swiss protP27361
SynonymsERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; p44-ERK1; p44-MAPK; MAPK3
Calculated MW38kDa/40kDa/43kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesDU145, HeLa, 293T
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - MAPK3 Rabbit pAb (A0228) }

Western blot - MAPK3 Rabbit pAb (A0228)

Western blot analysis of extracts of various cell lines, using MAPK3 antibody (A0228) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - MAPK3 Rabbit pAb (A0228) }

Western blot - MAPK3 Rabbit pAb (A0228)

Western blot analysis of extracts from normal (control) and MAPK3 Rabbit pAb knockout (KO) 293T cells, using MAPK3 Rabbit pAb antibody (A0228) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - MAPK3 Rabbit pAb (A0228) }

Immunohistochemistry - MAPK3 Rabbit pAb (A0228)

Immunohistochemistry of paraffin-embedded rat lung using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Rabbit pAb (A0228) }

Immunohistochemistry - MAPK3 Rabbit pAb (A0228)

Immunohistochemistry of paraffin-embedded human kidney using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAPK3 Rabbit pAb (A0228) }

Immunohistochemistry - MAPK3 Rabbit pAb (A0228)

Immunohistochemistry of paraffin-embedded mouse heart using MAPK3 Antibody (A0228) at dilution of 1:100 (40x lens).

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