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ERK1 / ERK2 Polyclonal Antibody (A16686)

Datasheet

ABclonal:Western blot - ERK1 / ERK2 Polyclonal Antibody (A16686)

Overview

Product nameERK1 / ERK2 Polyclonal Antibody
Catalog No.A16686
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant Protein of human ERK1 / ERK2
SequenceEmail for sequence
Gene ID5594/5595
Swiss protP28482/P27361
Synonyms
Calculated MW36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW42kDa/44kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:1000 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HepG2, 293T, mouse kidney, mouse brain, rat brain
Cellular location
ABclonal:Western blot - ERK1 / ERK2 Polyclonal Antibody (A16686) }

Western blot - ERK1 / ERK2 Polyclonal Antibody (A16686)

Western blot analysis of extracts of various cell lines, using ERK1 / ERK2 antibody (A16686) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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