Products > Catalog Antibodies > Polyclonal Antibodies

ERK1 / ERK2 Polyclonal Antibody (A16686)


ABclonal:Western blot - ERK1 / ERK2 Polyclonal Antibody (A16686)


Product nameERK1 / ERK2 Polyclonal Antibody
Catalog No.A16686
Host speciesRabbit
Purification methodAffinity purification
ImmunogenRecombinant Protein of human ERK1 / ERK2
SequenceEmail for sequence
Gene ID5594/5595
Swiss protP28482/P27361
Calculated MW36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW42kDa/44kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:1000 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HepG2, 293T, mouse kidney, mouse brain, rat brain
Cellular location
ABclonal:Western blot - ERK1 / ERK2 Polyclonal Antibody (A16686) }

Western blot - ERK1 / ERK2 Polyclonal Antibody (A16686)

Western blot analysis of extracts of various cell lines, using ERK1 / ERK2 antibody (A16686) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Submit an review for A16686

Please submit reviews to your technical sales specialist directly. Alternatively, email us at

Reviews List

Inquire About This Product

Submit your question about A16686 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
5 μl sample size available Add to Cart
Add to Cart

or Request quote for bulk purchase

Available for next day delivery (US only)

Contact us to order