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MAPK1/MAPK3 Monoclonal Antibody (A10613)

Publication (1) Datasheet

ABclonal:Western blot - MAPK1/MAPK3 antibody (A10613)

Overview

Product nameMAPK1/MAPK3 Monoclonal Antibody
Catalog No.A10613
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
ImmunogenA synthetic peptide of human MAPK1/MAPK3
SequenceEmail for sequence
Gene ID5594/5595
Swiss protP28482/P27361
Synonyms
Calculated MW36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW41kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:1000 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesJurkat, HepG2, A-431, NIH/3T3, Mouse brain, Mouse kidney, Mouse lung, Rat brain
Cellular location
Customer validation

WB(Human)

ABclonal:Western blot - MAPK1/MAPK3 antibody (A10613)}

Western blot - MAPK1/MAPK3 antibody (A10613)

Western blot analysis of extracts of various cell lines, using MAPK1/MAPK3 antibody (A10613) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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