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MAP4 Polyclonal Antibody (A5906)

Publication (1) Datasheet

ABclonal:Western blot - MAP4 Polyclonal Antibody (A5906)

Overview

Product nameMAP4 Polyclonal Antibody
Catalog No.A5906
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 942-1152 of human MAP4 (NP_002366.2).
SequenceTENIKHQPGGGRAKVEKKTEAAATTRKPESNAVTKTAGPIASAQKQPAGKVQIVSKKVSYSHIQSKCGSKDNIKHVPGGGNVQIQNKKVDISKVSSKCGSKANIKHKPGGGDVKIESQKLNFKEKAQAKVGSLDNVGHLPAGGAVKTEGGGSEAPLCPGPPAGEEPAISEAAPEAGAPTSASGLNGHPTLSGGGDQREAQTLDSQIQETSI
Gene ID4134
Swiss protP27816
SynonymsMAP4; microtubule-associated protein 4
Calculated MW10kDa/58kDa/85kDa/91kDa/102kDa/119kDa/121kDa
Observed MW200kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesU-87MG
Cellular locationCytoplasm, cytoskeleton
Customer validation

WB(Human)

ABclonal:Western blot - MAP4 Polyclonal Antibody (A5906) }

Western blot - MAP4 Polyclonal Antibody (A5906)

Western blot analysis of extracts of U-87MG cells, using MAP4 antibody (A5906) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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